LOCUS       Exported                4215 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRPGSM, complete sequence.
ACCESSION   EF380033
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Semsey S, Krishna S, Sneppen K, Adhya S.
  TITLE     Signal integration in the galactose network of Escherichia coli
  JOURNAL   Mol. Microbiol. 65 (2), 465-476 (2007)
  PUBMED    17630975
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Semsey S, Adhya S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2007) Genetics, Eotvos Lorand University, Pazmany 
            P. S. 1/C, Budapest H-1117, Hungary
REFERENCE   3  (bases 1 to 4215)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Cloning vector pRPGSM"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:436460"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     gene            160..1061
                     /gene="bla"
                     /label=bla
     regulatory      160..166
                     /regulatory_class="minus_10_signal"
                     /gene="bla"
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /protein_id="ABP51952.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1206..1879
                     /note="pMB1 origin; pUC derived"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(1917..1971)
                     /regulatory_class="terminator"
                     /note="rpoC terminator"
     misc_feature    complement(1977..2257)
                     /label=mgl promoter and regulatory region
                     /note="mgl promoter and regulatory region"
     regulatory      complement(2290..2344)
                     /regulatory_class="terminator"
                     /note="rpoC terminator"
     misc_feature    complement(2350..2649)
                     /label=galS promoter and regulatory region
                     /note="galS promoter and regulatory region"
     regulatory      complement(2682..2736)
                     /regulatory_class="terminator"
                     /note="rpoC terminator"
     misc_feature    complement(2737..3042)
                     /label=galETKM promoter and regulatory region
                     /note="galETKM promoter and regulatory region"
     regulatory      complement(3074..3128)
                     /regulatory_class="terminator"
                     /note="rpoC terminator"
     misc_feature    complement(3134..3463)
                     /label=galP promoter and regulatory region
                     /note="galP promoter and regulatory region"
     regulatory      complement(3486..3530)
                     /regulatory_class="terminator"
                     /note="rpoC terminator"
     misc_feature    complement(3536..3767)
                     /label=galR promoter and regulatory region
                     /note="galR promoter and regulatory region"
     primer_bind     complement(3819..3835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"