LOCUS       Exported                7686 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRP-M, complete sequence.
ACCESSION   KU315435
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Soysa R, Tran KD, Ullman B, Yates PA.
  TITLE     Integrating ribosomal promoter vectors that offer a choice of 
            constitutive expression profiles in Leishmania donovani
  JOURNAL   Mol. Biochem. Parasitol. (2016) In press
  PUBMED    26844641
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Yates PA, Ullman B, Soysa R.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2015) Biochemistry & Molecular Biology, Oregon 
            Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, 
            OR 97239, USA
REFERENCE   3  (bases 1 to 7686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Cloning vector pRP-M"
                     /mol_type="other DNA"
                     /db_xref="taxon:1796117"
     misc_feature    9..3185
                     /label=neomycin resistance cassette
                     /note="neomycin resistance cassette"
     CDS             981..1784
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /label=neomycin phosphotransferase
                     /protein_id="AMH38326.1"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1791..1839
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1962..1973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3205..5351
                     /note="minimal plasmid backbone; derived from pBluescript 
                     II"
     rep_origin      complement(3462..4050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4221..5081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5082..5186)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    5356..6003
                     /note="derived from Leishmania donovani ribosomal RNA 
                     spacer region"
     regulatory      6030..6579
                     /regulatory_class="promoter"
                     /note="derived from Leishmania donovani ribosomal RNA 
                     genes"
     misc_feature    6601..6682
                     /note="contains artificial trans-splice signal and 5-UTR; 
                     leishmanial consensus splice acceptor site; LdPARP"
     misc_feature    6689..6776
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    6787..7671
                     /note="derived from Leishmania major alpha tubulin 
                     intergenic region; LmTub"