LOCUS       Exported                8802 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRLSAYT9, complete sequence.
ACCESSION   JQ768520
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Redder P, Linder P.
  TITLE     New Range of Vectors with a Stringent 5-Fluoroorotic Acid-Based 
            Counterselection System for Generating Mutants by Allelic 
            Replacement in Staphylococcus aureus
  JOURNAL   Appl. Environ. Microbiol. 78 (11), 3846-3854 (2012)
  PUBMED    22447609
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Redder P, Linder P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2012) Department of Microbiology and Molecular 
            Medicine, University of Geneva, Faculty of Medicine, Rue 
            Michel-Servet 1, Geneva 1203, Switzerland
REFERENCE   3  (bases 1 to 8802)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit, CAP v. 7.0.9.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Cloning vector pRLSAYT9"
                     /mol_type="other DNA"
                     /db_xref="taxon:1193167"
     misc_feature    1..423
                     /label=dervied from pRLYT9
                     /note="dervied from pRLYT9"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    424..3788
                     /note="pyrFE genes from Staphylococcus aureus SA564 with 
                     flanking sequence; pyrFE region"
     misc_feature    3789..8802
                     /label=dervied from pRLYT9
                     /note="dervied from pRLYT9"
     misc_feature    complement(3789..5567)
                     /note="tetracyclin resistance cassette; tetK cassette"
     rep_origin      complement(5679..6842)
                     /direction=LEFT
                     /note="thermosensitive origin of replication; for 
                     replication in Staphylococcus aureus; pE194ts"
     rep_origin      6951..7565
                     /note="origin for replication in E. coli; ColE1"
     rep_origin      complement(6983..7571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7742..8602)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8603..8707)
                     /gene="bla"
                     /label=AmpR promoter