LOCUS       Exported                5844 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pRLE6, complete sequence.
ACCESSION   AY062236
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Priefert H, Achterholt S, Steinbuchel A.
  TITLE     Transformation of the Pseudonocardiaceae amycolatopsis sp. strain 
            HR167 is highly dependent on the physiological state of the cells
  JOURNAL   Appl. Microbiol. Biotechnol. 58 (4), 454-460 (2002)
  PUBMED    11954791
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Achterholt S, Priefert H, Steinbuechel A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2001) Institut fuer Mikrobiologie, Corrensstrasse 
            3, 48149 Muenster, Germany
REFERENCE   3  (bases 1 to 5844)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Transformation vector pRLE6"
                     /lab_host="Amycolatopsis sp. HR167"
                     /mol_type="other DNA"
                     /note="kanamycin-resistance gene and replication origin for
                     Amycolatopsis sp. derived from the plasmid pA387 (isolated 
                     from Amycolatopsis sp. DSM 43387) and a replication origin 
                     for Escherichia coli (derived from pBR322)."
                     /db_xref="taxon:181648"
     CDS             366..1160
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin/kanamycin-phosphotransferase-(type II)"
                     /label=nptII
                     /note="obtained from transposon Tn5"
                     /protein_id="AAL62335.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            366..1160
                     /gene="nptII"
                     /label=nptII
     rep_origin      complement(1791..2379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"