LOCUS       Exported                8850 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRL838, complete sequence.
ACCESSION   AF403425
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, Watanabe A, 
            Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M, 
            Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M, 
            Takazawa M, Yamada M, Yasuda M, Tabata S.
  TITLE     Complete genomic sequence of the filamentous nitrogen-fixing 
            cyanobacterium Anabaena sp. strain PCC 7120
  JOURNAL   DNA Res. 8 (5), 205-213 (2001)
  PUBMED    11759840
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan 
            State University, Wilson Road, E. Lansing, MI 48824-1312, USA
REFERENCE   3  (bases 1 to 8850)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Cloning vector pRL838"
                     /lab_host="Anabaena sp. strain PCC 7120"
                     /mol_type="other DNA"
                     /note="chloramphenicol- and erythromycin-resistant 
                     derivative of pBAC108L (GenBank Accession Number U51114)"
                     /db_xref="taxon:180220"
     misc_feature    1..5396
                     /label=pBAC108L (GenBank Accession Number U51114)
                     /note="pBAC108L (GenBank Accession Number U51114)"
     rep_origin      817..1036
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             1127..1882
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    1885..2135
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             2461..3636
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             3636..4607
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    4680..5153
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     oriT            5404..6172
                     /note="transfer origin oriT from RK2 (GenBank Accession 
                     Number L27758)"
     CDS             complement(5527..5898)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            5931..6040
                     /note="incP origin of transfer"
     misc_feature    6189..6591
                     /label=cos from lambda (NC_001416)
                     /note="cos from lambda (NC_001416)"
     misc_feature    6192..6591
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    6602..6635
                     /note="lox from pBAC108L (GenBank Accession Number U51114)"
     protein_bind    6602..6635
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      6651..6673
                     /regulatory_class="promoter"
                     /note="T7 promoter from pBAC108L (GenBank Accession Number 
                     U51114)"
     promoter        6651..6669
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6673..6832
                     /label=polylinker (multiple cloning site)
                     /note="polylinker (multiple cloning site)"
     regulatory      6832..6849
                     /regulatory_class="promoter"
                     /note="SP6 promoter from pBAC108L (GenBank Accession Number
                     U51114)"
     promoter        complement(6832..6849)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      6865..6891
                     /regulatory_class="promoter"
                     /note="cat promoter from pBR325 (GenBank Accession Number 
                     L08855)"
     gene            6958..7617
                     /gene="cat"
                     /label=cat
                     /note="from pBR325 (GenBank Accession Number L08855)"
     CDS             6958..7617
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            7871..8605
                     /gene="erm"
                     /label=erm
                     /note="from pE194 (GenBank Accession Number L08862)"
     terminator      8795..8838
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      8801..8829
                     /regulatory_class="terminator"
                     /note="rrnC terminator from Lorist6 (GenBank Accession 
                     Number X98450)"