LOCUS       Exported                6400 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Anabaena transfer vector pRL2665b, complete sequence.
ACCESSION   AY661563
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Huang G, Fan Q, Lechno-Yossef S, Wojciuch E, Wolk CP, Kaneko T, 
            Tabata S.
  TITLE     Clustered genes required for the synthesis of heterocyst envelope 
            polysaccharide in Anabaena sp. strain PCC 7120
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2004) MSU-DOE Plant Research Laboratory, Michigan 
            State University, Wilson Road, E. Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 6400)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2012) MSU-DOE Plant Research Laboratory, Michigan 
            State University, Wilson Road, E. Lansing, MI 48824, USA
REFERENCE   4  (bases 1 to 6400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Mar 1, 2012 this sequence version replaced AY661563.1.
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Anabaena transfer vector pRL2665b"
                     /mol_type="other DNA"
                     /note="permits conversion of sequencing clones borne in 
                     pUC18 and related plasmids to a form permitting their 
                     conjugal transfer to and selection in Anabaena sp. strain 
                     PCC 7120"
                     /db_xref="taxon:285601"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    560..700
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(886..1474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1758..2552)
                     /codon_start=1
                     /gene="npt"
                     /product="neomycin phosphotransferase"
                     /function="confers resistance to neomycin and kanamycin"
                     /label=npt
                     /note="derived from pK18 in GenBank Accesion Number M17626"
                     /protein_id="AAT74894.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(1758..2552)
                     /gene="npt"
                     /label=npt
     promoter        complement(3377..3481)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3788..3890
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     regulatory      3798..3824
                     /regulatory_class="promoter"
                     /note="cat promoter; derived from pBR325 in GenBank 
                     Accession Number L08855"
     CDS             3891..4550
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /function="confers resistance to chloramphenicol"
                     /label=cat
                     /note="derived from synthetic plasmid pBR325 in GenBank 
                     Accession Number L08855"
                     /protein_id="AAT74895.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            3891..4550
                     /gene="cat"
                     /label=cat
     CDS             4804..5538
                     /codon_start=1
                     /gene="erm"
                     /product="erythromycin-resistance protein"
                     /function="confers resistance to erythromycin"
                     /label=erm
                     /note="derived from pE194 in GenBank Accession Number 
                     L08862"
                     /protein_id="AAT74896.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
     gene            4804..5538
                     /gene="erm"
                     /label=erm
     oriT            5571..6346
                     /note="derived from RK2 (for conjugal transfer) in GenBank 
                     Accession Number L27758"
     CDS             complement(5697..6068)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6101..6210
                     /note="incP origin of transfer"