LOCUS       Exported                8416 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRL1342, complete sequence.
ACCESSION   AF403427
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Wolk CP.
  TITLE     pRL1342, an RSF1010-derived vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan 
            State University, Wilson Road, E. Lansing, MI 48824-1312, USA
REFERENCE   3  (bases 1 to 8416)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Cloning vector pRL1342"
                     /lab_host="Anabaena sp. strain PCC 7120"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:180218"
     misc_feature    1..5693
                     /note="pMMB66EH (GenBank Accession Number X15234), a 
                     derivative of RSF1010 (GenBank Accession Number M28829)"
     rep_origin      155..549
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            891..978
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2217..3188
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             3699..4538
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             4525..5376
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     regulatory      complement(5709..5735)
                     /regulatory_class="terminator"
                     /note="trpA terminator from Lorist6 (GenBank Accession 
                     Number X98450)"
     misc_feature    5755..6157
                     /label=cos from lambda (NC_001416)
                     /note="cos from lambda (NC_001416)"
     misc_feature    5758..6157
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    6168..6201
                     /note="lox from pBAC108L (GenBank Accession Number U51114)"
     protein_bind    6168..6201
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      6217..6239
                     /regulatory_class="promoter"
                     /note="T7 promoter from pBAC108L (GenBank Accession Number 
                     U51114)"
     promoter        6217..6235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6239..6398
                     /label=polylinker (multiple cloning site)
                     /note="polylinker (multiple cloning site)"
     regulatory      6398..6415
                     /regulatory_class="promoter"
                     /note="SP6 promoter from pBAC108L (GenBank Accession Number
                     U51114)"
     promoter        complement(6398..6415)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      6431..6457
                     /regulatory_class="promoter"
                     /note="cat promoter from pBR325 (GenBank Accession Number 
                     L08855)"
     gene            6524..7183
                     /gene="cat"
                     /label=cat
                     /note="from pBR325 (GenBank Accession Number L08855)"
     CDS             6524..7183
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            7437..8171
                     /gene="erm"
                     /label=erm
                     /note="from pE194 (GenBank Accession Number L08862)"
     terminator      8361..8404
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      8367..8395
                     /regulatory_class="terminator"
                     /note="rrnC terminator from Lorist6 (GenBank Accession 
                     Number X98450)"