LOCUS       Exported               11912 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range expression vector pRK415iq, complete sequence.
ACCESSION   EF435043
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Kickstein E, Harms K, Wackernagel W.
  TITLE     Deletions of recBCD or recD influence genetic transformation 
            differently and are lethal together with a recJ deletion in 
            Acinetobacter baylyi
  JOURNAL   Microbiology (Reading, Engl.) 153 (PT 7), 2259-2270 (2007)
  PUBMED    17600070
REFERENCE   2  (bases 1 to 11912)
  AUTHORS   Harms K, Huelter N, de Vries J, Wackernagel W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2007) Genetics, Department of Biology and 
            Environmental Sciences, University of Oldenburg, 
            Carl-von-Ossietzky-Strasse 9-11, Oldenburg D-26111, Germany
REFERENCE   3  (bases 1 to 11912)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11912
                     /organism="Broad host range expression vector pRK415iq"
                     /mol_type="other DNA"
                     /db_xref="taxon:429721"
     rep_origin      1..712
                     /label=IncP alpha
                     /note="IncP alpha"
     CDS             complement(971..1312)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase alpha protein"
                     /label=lacZ
                     /protein_id="ABO32164.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRHASRREKGGQVSGKRQGRNRRAHEGA
                     SRGKRLVSL"
     gene            complement(971..1312)
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     1222..1238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1239..1295
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(1308..1324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1332..1348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(1352..1421)
                     /regulatory_class="promoter"
                     /note="lac"
     promoter        complement(1356..1386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1401..1422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(1654..2025)
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ protein"
                     /label=traJ
                     /protein_id="ABO32165.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     gene            complement(1654..2025)
                     /gene="traJ"
                     /label=traJ
     oriT            2058..2167
     CDS             complement(2848..3498)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulation protein"
                     /label=tetR
                     /protein_id="ABO32166.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(2848..3498)
                     /gene="tetR"
                     /label=tetR
     CDS             3604..4803
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline resistance protein"
                     /label=tetA
                     /protein_id="ABO32167.1"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     gene            3604..4803
                     /gene="tetA"
                     /label=tetA
     promoter        4840..4917
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     gene            4852..6000
                     /gene="lacI"
                     /label=lacI
     regulatory      4852..4883
                     /regulatory_class="promoter"
                     /gene="lacI"
                     /note="lacIq promoter"
     misc_difference 4855
                     /gene="lacI"
                     /label=lacIq point mutation
                     /note="lacIq point mutation"
     CDS             4918..6000
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor protein"
                     /label=lacI
                     /protein_id="ABO32168.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             complement(6656..7321)
                     /codon_start=1
                     /gene="upf16.5"
                     /product="unknown plasmid factor 16.5"
                     /label=upf16.5
                     /protein_id="ABO32169.1"
                     /translation="MTWCIANARALWGQFRLGVQQPALYWHFRNKRALLDALASLSIAR
                     AHGALYELPINRGLKLTIVIKAQIRRLGAADVQDFREIRLSALKKAPEMFGSVYEHEEK
                     KPMEAFAERLRDAVAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVA
                     GMLLRALPAGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGSRRR"
     gene            complement(6656..7321)
                     /gene="upf16.5"
                     /label=upf16.5
     CDS             complement(7296..8444)
                     /codon_start=1
                     /gene="trfA2"
                     /product="trans-acting replication A2"
                     /label=trfA2
                     /protein_id="ABO32170.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            complement(7296..8444)
                     /gene="trfA2"
                     /label=trfA2
     CDS             complement(7296..8153)
                     /codon_start=1
                     /gene="trfA1"
                     /product="trans-acting replication A1"
                     /label=trfA1
                     /protein_id="ABO32171.1"
                     /translation="MTTKKRKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAP
                     WPDTMRAMPNDTARSALFTTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELV
                     WQQVLEYAKRTPIGEPITFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRV
                     GHLESVSLLHRFRVLDRGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRM
                     FDYFSSHREPYPLKLETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLV
                     HCKR"
     gene            complement(7296..8153)
                     /gene="trfA1"
                     /label=trfA1
     CDS             complement(8493..8843)
                     /codon_start=1
                     /gene="ssb"
                     /product="single-strand binding protein"
                     /label=ssb
                     /protein_id="ABO32172.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(8493..8843)
                     /gene="ssb"
                     /label=ssb
     CDS             8964..9329
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label=trbA
                     /note="regulation of tra gene expression"
                     /protein_id="ABO32173.1"
                     /translation="MYNQIFFTNILRLLDERGMTKHELSERAGVSISFLSDLTNGKANP
                     SLKVMEAIADALETPLPLLLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAF
                     IVKKWGDDTRKKLRGRL"
     gene            8964..9329
                     /gene="trbA"
                     /label=trbA
     CDS             complement(10367..11461)
                     /codon_start=1
                     /gene="incC1"
                     /product="IncC1"
                     /label=incC1
                     /note="IncC partitioning protein, long form"
                     /protein_id="ABO32174.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(10367..11461)
                     /gene="incC1"
                     /label=incC1
     CDS             complement(10367..11146)
                     /codon_start=1
                     /gene="incC2"
                     /product="IncC2"
                     /label=incC2
                     /note="IncC partitioning protein, short form"
                     /protein_id="ABO32175.1"
                     /translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
                     YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
                     RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
                     TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
                     LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
     gene            complement(10367..11146)
                     /gene="incC2"
                     /label=incC2
     CDS             complement(11143..11448)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label=korA
                     /note="regulation of IncC repression"
                     /protein_id="ABO32176.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(11143..11448)
                     /gene="korA"
                     /label=korA