LOCUS       Exported                5241 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRIE, complete sequence.
ACCESSION   JQ638951
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Welch MD, Reed SC, Lamason RL, Serio AW.
  TITLE     Expression of an Epitope-Tagged Virulence Protein in Rickettsia 
            parkeri Using Transposon Insertion
  JOURNAL   PLoS ONE 7 (5), E37310 (2012)
  PUBMED    22624012
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Reed SCO., Welch MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2012) Molecular and Cell Biology, University of 
            California, Berkeley, 305 Life Sciences Addition, Berkeley, CA 
            94720-3200, USA
REFERENCE   3  (bases 1 to 5241)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Cloning vector pRIE"
                     /mol_type="other DNA"
                     /db_xref="taxon:1184578"
     repeat_region   7..5214
                     /label=ITR2
                     /note="ITR2"
     protein_bind    112..133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        148..178
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    186..202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     210..226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      495..671
                     /regulatory_class="promoter"
                     /note="flgBp"
     CDS             672..1718
                     /codon_start=1
                     /product="Himar1"
                     /label=Himar1
                     /note="Mariner transposase"
                     /protein_id="AFJ44823.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQQRVDDSERCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIRELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYRNGIKKLEGRYNRCI
                     ALEGNYVE"
     CDS             2092..2901
                     /codon_start=1
                     /product="KanR"
                     /label=KanR
                     /note="kanamycin resistance protein"
                     /protein_id="AFJ44821.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2986..3002)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3143..3178
                     /label=ITR1
                     /note="ITR1"
     rep_origin      complement(3279..3867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4076..4175
                     /label=MCS2
                     /note="MCS2"
     misc_feature    complement(4408..4431)
                     /label=MCS1
                     /note="MCS1"
     regulatory      complement(4432..4572)
                     /regulatory_class="promoter"
                     /note="OmpAp"
     CDS             complement(4591..5043)
                     /codon_start=1
                     /product="Rparr-2"
                     /label=Rparr-2
                     /note="rifampicin resistance protein"
                     /protein_id="AFJ44822.1"
                     /translation="MVKDWIPISHDNYKQVQGPFYHGTKANLAIGDLLTTGFISHFEDG
                     RILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDDPNLTNKKFPGNPT
                     QSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED"
     regulatory      complement(5044..5204)
                     /regulatory_class="promoter"
                     /note="rpsLp"