LOCUS       Exported               11241 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pRHBR17, complete sequence.
ACCESSION   AY180162
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Kostichka K, Tao L, Bramucci M, Tomb JF, Nagarajan V, Cheng Q.
  TITLE     A small cryptic plasmid from Rhodococcus erythropolis: 
            characterization and utility for gene expression
  JOURNAL   Appl. Microbiol. Biotechnol. 62 (1), 61-68 (2003)
  PUBMED    12835922
REFERENCE   2  (bases 1 to 11241)
  AUTHORS   Kostichka K, Tao L, Brammucci M, Tomb J-F., Nagarajan V, Cheng Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2002) CR@D, E. I. Dupont de Numours Inc., 
            Experimental Station, Wilmington, DE 19880-0328, USA
REFERENCE   3  (bases 1 to 11241)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Shuttle vector pRHBR17"
                     /focus
                     /mol_type="genomic DNA"
                     /note="Rhodococcus - Escherichia coli shuttle vector; Ssp I
                     linearize pAN12 cloned into the PvuII site of pBR328"
                     /db_xref="taxon:214938"
     source          3591..9924
                     /organism="Rhodococcus erythropolis"
                     /plasmid="pAN12"
                     /mol_type="genomic DNA"
                     /label=cryptic plasmid
                     /note="cryptic plasmid"
                     /db_xref="taxon:1833"
     source          9925..3590
                     /organism="Cloning vector pBR328"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:221946"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             86..1276
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     rep_origin      complement(1447..2035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2206..3066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3067..3171)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3374..3476
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(8850..8861)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"