LOCUS       Exported                7260 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pRESQ70, complete sequence.
ACCESSION   EU503043
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Sivamani E, Qu R.
  TITLE     Expression enhancement of a rice polyubiquitin gene promoter
  JOURNAL   Plant Mol. Biol. 60 (2), 225-239 (2006)
  PUBMED    16429261
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Sivamani E, Starmer J, Qu R.
  TITLE     Sequence analysis of rice rubi3 promoter gene expression cassettes 
            for improved transgene expression
  JOURNAL   Plant Sci. 177 (6), 549-556 (2009)
REFERENCE   3  (bases 1 to 7260)
  AUTHORS   Sivamani E, Qu R.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-FEB-2008) Crop Science, North Carolina State 
            University, 101, Derieux Place, Raleigh, NC 27695, USA
REFERENCE   4  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Expression vector pRESQ70"
                     /mol_type="other DNA"
                     /label=monocot expression vector
                     /note="monocot expression vector"
                     /db_xref="taxon:510745"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            325..2315
                     /gene="rubi3"
                     /label=rubi3
                     /note="polyubiquitin; from rice"
     regulatory      325..1108
                     /regulatory_class="promoter"
                     /gene="rubi3"
     exon            1109..1175
                     /gene="rubi3"
                     /number=1
     intron          1176..2315
                     /gene="rubi3"
                     /number=1
     CDS             2316..4127
                     /codon_start=1
                     /gene="GUS"
                     /product="GUS"
                     /label=GUS
                     /note="reporter"
                     /protein_id="ACA96990.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            2316..4127
                     /gene="GUS"
                     /label=GUS
     regulatory      4128..4383
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      4128..4380
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4389..4405)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4618..5073
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5355..5459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5460..6320
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR; ampicillin-resistance protein"
                     /protein_id="ACA96991.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6491..7079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"