LOCUS       Exported               10928 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRESC800, complete sequence.
ACCESSION   JQ354897
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Gase K, Baldwin IT.
  TITLE     Transformational tools for next-generation plant ecology: 
            manipulation of gene expression for the functional analysis of genes
  JOURNAL   Plant Ecol Divers 5 (4), 485-490 (2012)
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Gase K, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2012) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, 
            Germany
REFERENCE   3  (bases 1 to 10928)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Cloning vector pRESC800"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246479"
     misc_feature    244..268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    244..266
                     /label=left border T-DNA
                     /note="left border T-DNA"
     regulatory      complement(335..612)
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      342..594
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(616..1641)
                     /codon_start=1
                     /product="hygromycin phosphotransferase II"
                     /label=hygromycin phosphotransferase II
                     /note="HptII"
                     /protein_id="AFV47151.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     regulatory      complement(1642..1960)
                     /regulatory_class="promoter"
                     /note="NOS"
     promoter        complement(1662..1845)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1698..1877)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      1967..2514
                     /regulatory_class="promoter"
                     /note="35S cauliflower mosaic virus"
     promoter        2155..2500
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     stem_loop       join(2521..2727,3519..3725)
                     /note="Nicotiana benthamiana pds gene fragment; INSD 
                     accession AJ571700"
     intron          2738..3506
                     /note="Flaveria trinervia pyruvate, orthophosphate 
                     dikinase; INSD accession X79095; intron 3"
     regulatory      3739..3930
                     /regulatory_class="terminator"
                     /note="35S cauliflower mosaic virus"
     polyA_signal    3744..3918
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        3962..4066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4067..4927
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /note="bla; pUC19 INSD accession L09137"
                     /protein_id="AFV47152.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4855..5747
                     /note="ColE1; pUC19; INSD accession L09137"
     rep_origin      5098..5686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5806..5831
                     /label=right border T-DNA
                     /note="right border T-DNA"
     misc_feature    5807..5831
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6327..6521
                     /label=pVS1
                     /note="pVS1"
     CDS             complement(6587..7762)
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /note="RepA; pVS1"
                     /protein_id="AFV47153.1"
                     /translation="MPHVWRNGRLARRKRLGCLPALQWHWNPQARGIGVSGRKPSGPVQ
                     IGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKP
                     PKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGP
                     AGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKD
                     DLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELAD
                     WMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSA
                     GEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
     CDS             complement(6587..7660)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8089..8718)
                     /codon_start=1
                     /product="partitioning protein"
                     /label=partitioning protein
                     /note="StaA; pVS1"
                     /protein_id="AFV47154.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10128..10922
                     /codon_start=1
                     /product="neomycin phosphotransferase class II"
                     /label=neomycin phosphotransferase class II
                     /note="NptII"
                     /protein_id="AFV47155.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"