LOCUS       Exported               11150 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRESC5GGPPS, complete sequence.
ACCESSION   KX011462
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Gase K, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, 
            Germany
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Cloning vector pRESC5GGPPS"
                     /mol_type="other DNA"
                     /db_xref="taxon:1844593"
     misc_feature    244..268
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    244..266
                     /label=left border T-DNA
                     /note="left border T-DNA"
     regulatory      complement(335..612)
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      342..594
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(616..1641)
                     /codon_start=1
                     /product="hygromycin phosphotransferase II"
                     /label=hygromycin phosphotransferase II
                     /note="HptII"
                     /protein_id="ANR01675.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     regulatory      complement(1642..1960)
                     /regulatory_class="promoter"
                     /note="NOS"
     promoter        complement(1662..1845)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(1698..1877)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      1967..2514
                     /regulatory_class="promoter"
                     /note="35S cauliflower mosaic virus"
     promoter        2155..2500
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     stem_loop       join(2521..2838,3630..3947)
                     /note="Nicotiana attenuata geranylgeranyl pyrophosphate 
                     synthase (ggpps) gene fragment; INSD accession EF382626"
     intron          2849..3617
                     /note="Flaveria trinervia pyruvate, orthophosphate 
                     dikinase; INSD accession X79095; intron 3"
     regulatory      3961..4152
                     /regulatory_class="terminator"
                     /note="35S cauliflower mosaic virus"
     polyA_signal    3966..4140
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4184..4288
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4289..5149
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /note="bla; pUC19 INSD accession L09137"
                     /protein_id="ANR01676.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5077..5969
                     /note="ColE1; pUC19; INSD accession L09137"
     rep_origin      5320..5908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6028..6053
                     /label=right border T-DNA
                     /note="right border T-DNA"
     misc_feature    6029..6053
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6549..6743
                     /label=pVS1
                     /note="pVS1"
     CDS             complement(6809..7984)
                     /codon_start=1
                     /product="replication protein"
                     /label=replication protein
                     /note="RepA; pVS1"
                     /protein_id="ANR01677.1"
                     /translation="MPHVWRNGRLARRKRLGCLPALQWHWNPQARGIGVSGRKPSGPVQ
                     IGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKP
                     PKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGP
                     AGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKD
                     DLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELAD
                     WMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSA
                     GEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
     CDS             complement(6809..7882)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8311..8940)
                     /codon_start=1
                     /product="partitioning protein"
                     /label=partitioning protein
                     /note="StaA; pVS1"
                     /protein_id="ANR01678.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10350..11144
                     /codon_start=1
                     /product="neomycin phosphotransferase class III"
                     /label=neomycin phosphotransferase class III
                     /note="NptIII"
                     /protein_id="ANR01679.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"