LOCUS       Exported                6140 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pRES19 DNA, complete sequence.
ACCESSION   AB040452
VERSION     .
KEYWORDS    beta-galactosidase; beta-lactamase; rRNA methylase; rep protein.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Niino Y, Hotta K.
  TITLE     Construction of pRES18 and pRES19, Streptomyces-Escherichia coli 
            shuttle vectors carrying multiple cloning sites
  JOURNAL   FEMS Microbiol. Lett. 145 (1), 113-116 (1996)
  PUBMED    8931335
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Sudoh M, Hotta K.
  TITLE     Nucleotide sequence of the pRES19
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Sudoh M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2000) Jun Ishikawa, National Institute of 
            Infectious Diseases, Department of Bioactive Molecules; 1-23-1 
            Toyama, Shinjuku, Tokyo 162-8640, Japan (E-mail:jun@nih.go.jp, 
            URL:http://www.nih.go.jp/niid/index-e.html, 
            Tel:81-3-5285-1111(ex.2354), Fax:81-3-5285-1272)
REFERENCE   4  (bases 1 to 6140)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Shuttle vector pRES19"
                     /focus
                     /lab_host="Streptomyces griseus"
                     /mol_type="other DNA"
                     /label=Streptomyces-Escherichia coli shuttle vector
                     /note="Streptomyces-Escherichia coli shuttle vector"
                     /db_xref="taxon:119636"
     source          2370..5055
                     /organism="Cloning vector pUC19"
                     /mol_type="other DNA"
                     /db_xref="taxon:31851"
     regulatory      425..434
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(1216..2025)
                     /codon_start=1
                     /gene="tsr"
                     /product="rRNA methylase"
                     /label=tsr
                     /protein_id="BAA93040.1"
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     gene            complement(1216..2025)
                     /gene="tsr"
                     /label=tsr
     promoter        2703..2807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2808..3668
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="BAA93041.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2808..3668
                     /gene="bla"
                     /label=bla
     rep_origin      3839..4427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4715..4736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4751..4781
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4789..4805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4813..4829
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4825..5103
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /protein_id="BAA93042.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRVEGPLPVSGGGPFL"
     gene            4825..5103
                     /gene="lacZ"
                     /label=lacZ
     misc_feature    4842..4898
                     /gene="lacZ"
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     primer_bind     complement(4899..4915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5287..175)
                     /codon_start=1
                     /gene="rep"
                     /product="rep protein"
                     /label=rep
                     /note="probable"
                     /protein_id="BAA93043.1"
                     /translation="MAQGQNGKVDRVKIHVRDGVAYTSGLCICANGWLCPVCSAKIRAK
                     RANEIAVGVASLIMGGGEAWMVTFTVRHARGHALADLLNALNDAFRKLGTGVMAVREKK
                     ATGKIGTINSREITYGKNGWHPHLHVIVCFKEVPDVRELAIMMGRWQRLWMAWTKKHGF
                     PADKAHGVKWDKVMTAEGAGEYIAKAQDSGKHIGNEVARGDLKKGKLGSLTPFEMIEYL
                     MATGDAAVVDLWLEYEKATKGKSWLRWSPKLKAMLGVDEKTEEELAAEEVAGGEVAELA
                     EAAWKMVIRHGLEARVLEAVERGGFPALVKLLTAHQIWAVKKLTPDDASETPDGPSESN
                     AA"
     gene            complement(5287..175)
                     /gene="rep"
                     /label=rep