LOCUS       Exported                8482 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pRES, complete sequence.
ACCESSION   KF498961
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Hu Q, Miao S, Ni X, Lu F, Yu H, Xing L, Jiang P.
  TITLE     Construction of a shuttle vector for use in Riemerella anatipestifer
  JOURNAL   J. Microbiol. Methods 95 (2), 262-267 (2013)
  PUBMED    24064367
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Hu Q, Miao S, Ni X, Lu F, Yu H, Xing L, Jiang P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2013) Poultry Diseases Research, Shanghai 
            Veterinary Research Institute, CAAS, No. 518, Ziyue Road, Shanghai, 
            Shanghai 200241, China
REFERENCE   3  (bases 1 to 8482)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Shuttle vector pRES"
                     /mol_type="other DNA"
                     /db_xref="taxon:1527691"
     regulatory      1..266
                     /regulatory_class="promoter"
                     /note="putative streptothricin resistance gene"
     primer_bind     complement(341..357)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    365..381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(389..419)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    434..455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(743..1331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1502..2362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(1502..2167)
                     /codon_start=1
                     /gene="bla"
                     /product="ampicillin resistance protein"
                     /label=bla
                     /protein_id="AII17663.1"
                     /translation="MMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
                     LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNE
                     AIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWF
                     IADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     gene            complement(1502..2167)
                     /gene="bla"
                     /label=bla
     promoter        complement(2363..2467)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(6046..6417)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6450..6559
                     /note="incP origin of transfer"
     CDS             6858..7823
                     /codon_start=1
                     /gene="cfxA"
                     /product="cefoxitin resistance protein"
                     /label=cfxA
                     /protein_id="AII17664.1"
                     /translation="MEKNRKKQIVVLSIALVCIFILVFSLFHKSATKDSANPPLTNVLT
                     DSISQIVSACPGEIGVAVIVNNRDTVKVNNKSVYPMMSVFKVHQALALCNDFDNKGISL
                     DTLVNINRDKLDPKTWSPMLKDYSGPVISLTVRDLLRYTLTQSDNNASNLMFKDMVNVA
                     QTDSFIATLIPRSSFQIAYTEEEMSADHNKAYSNYTSPLGAAMLMNRLFTEGLIDDEKQ
                     SFIKNTLKECKTGVDRIAAPLLDKEGVVIAHKTGSGYVNENGVLAAHNDVAYICLPNNI
                     SYTLAVFVKDFKGNKSQASQYVAHISAVVYSLLMQTSVKS"
     gene            6858..7823
                     /gene="cfxA"
                     /label=cfxA