LOCUS       Exported                9769 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pRE1, complete sequence.
ACCESSION   AY456904
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Toepfer R, Filsak E, Hausmann L.
  TITLE     Binary vector for agrobacterium-mediated plant transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Toepfer R, Filsak E, Hausmann L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2003) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Binary vector pRE1"
                     /mol_type="other DNA"
                     /db_xref="taxon:255362"
     misc_feature    1..102
                     /note="multiple cloning site from vector pBluescript; 
                     GenBank Accession Number: X52324"
     primer_bind     12..28
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(62..78)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(117..316)
                     /regulatory_class="terminator"
                     /note="terminator sequence from the 35S gene of Cauliflower
                     mosaic virus; GenBank Accession Number: X05868, V00140"
     misc_feature    complement(121)
                     /label=putative transcription stop site
                     /note="putative transcription stop site"
     regulatory      complement(136..151)
                     /regulatory_class="polyA_signal_sequence"
     misc_difference 278
                     /label=compared to GenBank Accession Number X05868
                     /note="compared to GenBank Accession Number X05868"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     gene            complement(323..1133)
                     /gene="nptII"
                     /label=nptII
                     /note="neomycin/kanamycin resistance gene of Tn5 from 
                     Escherichia coli; similar to GenBank Accession Number 
                     U00004"
     CDS             complement(330..1133)
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin phosphotransferase"
                     /EC_number="2.7.1.95"
                     /label=nptII
                     /note="NPTII; confers resistance to kanamycin and neomycin"
                     /protein_id="AAR20818.1"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(330..1121)
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      1130..1139
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      1148..1566
                     /regulatory_class="promoter"
                     /note="promoter/enhancer sequence from the 35S gene of 
                     Cauliflower mosaic virus: GenBank Accession Number X05868, 
                     M31331"
     promoter        complement(1148..1492)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    complement(1149)
                     /label=putative transcription start site
                     /note="putative transcription start site"
     regulatory      complement(1174..1180)
                     /regulatory_class="TATA_box"
     regulatory      complement(1209..1212)
                     /regulatory_class="CAAT_signal"
     misc_feature    1598..2617
                     /note="left border region from octopine-type Ti plasmid of 
                     Agrobacterium tumefaciens; similar to GenBank Accession 
                     Number X00493"
     misc_difference 1883
                     /replace="g"
                     /label=compared to GenBank Accession Number X00493
                     /note="compared to GenBank Accession Number X00493"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    2288..2312
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    2288..2311
                     /label=left flanking T-DNA border
                     /note="left flanking T-DNA border"
     misc_feature    2618..4382
                     /label=similar to GenBank Accession Number X12870, M35007
                     /note="similar to GenBank Accession Number X12870, M35007"
     stem_loop       complement(2810..2867)
                     /note="palindromic sequence; putative termination signal"
     CDS             complement(2870..3880)
                     /codon_start=1
                     /gene="aadA"
                     /product="streptomycin/spectinomycin adenyltransferase"
                     /EC_number="2.7.7.47"
                     /label=aadA
                     /note="confers resistance to streptomycin and 
                     spectinomycin; different start codons are described in the 
                     literature"
                     /protein_id="AAR20819.1"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2870..3880)
                     /gene="aadA"
                     /label=aadA
     CDS             complement(2870..3661)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_difference 3350
                     /replace="c"
                     /note="compared to GenBank Accession Number X12870, M35007"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     regulatory      complement(3897..3902)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(3920..3925)
                     /regulatory_class="minus_35_signal"
     rep_origin      4387..8158
                     /note="origin of replication and partitioning region from 
                     plasmid pVS1 of Pseudomonas (Itoh et al. 1984, Plasmid 11: 
                     206-220); similar to GenBank Accession Number U10487; 
                     serves as origin of replication in Agrobacterium 
                     tumefaciens host"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     regulatory      4518..4523
                     /regulatory_class="minus_35_signal"
     regulatory      4544..4549
                     /regulatory_class="minus_10_signal"
     regulatory      4566..4570
                     /regulatory_class="ribosome_binding_site"
     CDS             4580..5266
                     /codon_start=1
                     /product="resolvase"
                     /label=resolvase
                     /note="resolvase-like protein encoded by parR of plasmid 
                     pVS1; similar to Tn3 resolvase in GenBank Accession Number 
                     V00613, Tn917 resolvase in GenBank Accession Number M11180,
                     and RK2 ParA in GenBank Accession Number L27758"
                     /protein_id="AAR20820.1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT
                     RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT
                     TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI
                     DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE
                     RQEEQA"
     stem_loop       5410..5442
                     /label=palindromic sequence
                     /note="palindromic sequence"
     regulatory      5500..5505
                     /regulatory_class="minus_35_signal"
     regulatory      5521..5526
                     /regulatory_class="minus_10_signal"
     regulatory      5554..5561
                     /regulatory_class="ribosome_binding_site"
     CDS             5565..6194
                     /codon_start=1
                     /product="ParA"
                     /label=ParA
                     /note="partitioning protein ParA encoded by parA of plasmid
                     pVS1; similar to F plasmid SopA in GenBank Accession Number
                     X04619, RK2 IncC in GenBank Accession Number L27758, and P1
                     ParA in GenBank Accession Number X02954"
                     /protein_id="AAR20821.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6461..6473
                     /note="identical to the korB operator binding site of 
                     plasmid RK2 (Pansegrau et al. 1994, J. Mol. Biol. 239: 
                     623-663) in GenBank Accession Number L27758"
     CDS             6521..7696
                     /codon_start=1
                     /product="ParB"
                     /label=ParB
                     /note="partitioning protein ParB encoded by parB of plasmid
                     pVS1; similar to F plasmid SopB in GenBank Accession Number
                     X04619, RK2 KorB in GenBank Accession Number L27758, and P1
                     ParB in GenBank Accession Number X02954"
                     /protein_id="AAR20822.1"
                     /translation="MPHVWRNGRLARRKRLGCLPALQWHWNPQARGIGVSGRKPSGPVQ
                     IGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKP
                     PKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGP
                     AGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKD
                     DLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELAD
                     WMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSA
                     GEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS"
     CDS             6623..7696
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     misc_difference 6627
                     /label=compared to GenBank Accession Number U10487
                     /note="compared to GenBank Accession Number U10487"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     repeat_region   7688..7702
                     /label=repeat D
                     /note="repeat D"
     stem_loop       7710..7732
                     /label=palindromic sequence
                     /note="palindromic sequence"
     misc_feature    7739..8158
                     /note="parC region; contains putative cis-acting sites for 
                     replication, partition and incompatibility"
     rep_origin      7762..7956
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     protein_bind    7762..7770
                     /label=DnaA binding site
                     /bound_moiety="DnaA"
                     /note="putative DnaA box"
     repeat_region   7773..7787
                     /label=repeat D
                     /note="repeat D"
     repeat_region   7794..7808
                     /label=repeat D
                     /note="repeat D"
     repeat_region   7815..7829
                     /label=repeat D
                     /note="repeat D"
     repeat_region   7836..7850
                     /label=repeat D
                     /note="repeat D"
     misc_feature    7859..7937
                     /label=AT-rich region
                     /note="AT-rich region"
     protein_bind    7949..7957
                     /label=DnaA binding site
                     /bound_moiety="DnaA"
                     /note="putative DnaA box"
     repeat_region   8053..8066
                     /label=repeat E
                     /rpt_unit_range=8053 .. 8059
                     /note="repeat E"
     rep_origin      complement(8159..9222)
                     /direction=LEFT
                     /note="ColE1 origin of replication sequence of pBR322; 
                     similar to GenBank Accession Number J01749; serves as 
                     origin of replication in Escherichia coli host"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    8300..8440
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    8347
                     /label=nic/bom site
                     /note="nic/bom site"
     rep_origin      complement(8626..9214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_difference 9123
                     /replace="g"
                     /label=compared to GenBank Accession Number J01749
                     /note="compared to GenBank Accession Number J01749"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    9330..9769
                     /note="right border region from octopine-type Ti plasmid of
                     Agrobacterium tumefaciens in GenBank Accession Number 
                     X07435"
     misc_feature    9485..9508
                     /label=overdrive sequence
                     /note="overdrive sequence"
     misc_feature    9523..9547
                     /label=RB T-DNA repeat
                     /note="right border repeat from octopine Ach5 T-DNA"
     misc_feature    9523..9546
                     /label=right flanking T-DNA border
                     /note="right flanking T-DNA border"