LOCUS       Exported                4899 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector prCol2(P)-FRT DNA, complete sequence.
ACCESSION   LC089713
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ito K, Shinomura T.
  TITLE     Development and application of a new Silent reporter system to 
            quantitate the activity of enhancer elements in the type II Collagen
            Gene
  JOURNAL   Gene (2016) In press
  PUBMED    26992640
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Contact:Tamayuki Shinomura Tokyo Medical and
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan
REFERENCE   3  (bases 1 to 4899)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Expression vector prCol2(P)-FRT"
                     /mol_type="other DNA"
                     /db_xref="taxon:1735610"
     promoter        14..32
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     gene            78..1727
                     /gene="Col2a1"
                     /label=Col2a1
     regulatory      78..1066
                     /regulatory_class="promoter"
                     /gene="Col2a1"
                     /note="rat type II collagen"
     exon            1067..1307
                     /gene="Col2a1"
                     /number=1
                     /note="rat type II collagen"
     intron          1308..1351
                     /gene="Col2a1"
                     /number=1
                     /note="rat type II collagen partial intron"
     intron          1556..1710
                     /gene="Col2a1"
                     /number=2
                     /note="rat type II collagen partial intron"
     exon            1711..1727
                     /gene="Col2a1"
                     /number=3
                     /note="rat type II collagen partial exon"
     misc_feature    1736..1783
                     /label=FRT (FLP recombination target)
                     /note="FRT (FLP recombination target)"
     protein_bind    1736..1783
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(1798..1816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1823..1839)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1980..2435)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2769..2873
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3905..4493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4781..4802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4817..4847
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4855..4871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4879..4895
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"