LOCUS       Exported                4566 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRC-Niv-N, complete sequence.
ACCESSION   DQ508095
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P.
  TITLE     Establishment of a Taqman real time RT-PCR assay for the detection 
            of Nipah virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2006) Chinese Center for Animal Health and 
            Epidemiology, Nanjing Road 369, Qingdao, Shandong 266032, China
REFERENCE   3  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Cloning vector pRC-Niv-N"
                     /mol_type="other DNA"
                     /db_xref="taxon:394040"
     CDS             23..1621
                     /codon_start=1
                     /product="Nipah virus N protein"
                     /label=Nipah virus N protein
                     /protein_id="ABG78998.1"
                     /translation="MSDIFEEAASFRSYQSKLGRDGRASAATATLTTKIRIFVPATNSP
                     ELRWELTLFALDVIRSPSAAESMKVGAAFTLISMYSERPGALIRSLLNDPDIEAVIIDV
                     GSMVNGIPVMERRGDKAQEEMEGLMRILKTARDSSKGKTPFVDSRAYGLRITDMSTLVS
                     AVITIEAQIWILIAKAVTAPDTAEESETRRWAKYVQQKRVNPFFALTQQWLTEMRNLLS
                     QSLSVRKFMVEILIEVKKGGSAKGRAVEIISDIGNYVEETGMAGFFATIRFGLETRYPA
                     LALNEFQSDLNTIKSLMLLYREIGPRAPYMVLLEESIQTKFAPGGYPLLWSFAMGVATT
                     IDRSMGALNINRGYLEPMYFRLGQKSARHHAGGIDQNMANRLGLSSDQVAELAAAVQET
                     SAGRQESNVQAREAKFAAGGVLIGGSDQDIDEGEEPIEQSGRQSVTFKREMSISSLANS
                     VPSSSVSTSGGTRLTNSLLNLRSRLAAKAAKEAASSNATDDPAISNRTQGESEKKNNQD
                     LKPAQNDLDFVRADV"
     terminator      1728..1775
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1872..2327
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2408..2512
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3544..4132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4387..4405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4436..4458
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4478..4495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4499..4531
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             4535..4558
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"