LOCUS       Exported                5312 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRama-35, complete sequence.
ACCESSION   GQ258350
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ganguly S, Liu J, Pillai VB, Mittler RS, Amara RR.
  TITLE     Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a 
            HIV-1 Gag DNA vaccine: different effects on cellular and humoral 
            immunity
  JOURNAL   Vaccine 28 (5), 1300-1309 (2010)
  PUBMED    19944789
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Pillai VB, Amara RR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2009) Microbiology and Immunology, Emory 
            University, 954 Gatewood Road NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 5312)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Cloning vector pRama-35"
                     /mol_type="other DNA"
                     /db_xref="taxon:658873"
     regulatory      1..690
                     /regulatory_class="promoter"
                     /note="CMV immediate early promoter"
     enhancer        93..474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          691..1638
                     /note="CMV intron A"
     intron          814..1640
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             1784..3172
                     /codon_start=1
                     /product="SP-D-4-1BBL"
                     /label=SP-D-4-1BBL
                     /note="multimerization domain of lung surfactant protein D 
                     (SP-D) is fused to the extracellular domain of 4-1BBL"
                     /protein_id="ACT68289.1"
                     /translation="MLPFLSMLVLLVQPLGNLGAEMKSLSQRSVPNTCTLVMCSPTENG
                     LPGRDGRDGREGPRGEKGDPGLPGPMGLSGLQGPTGPVGPKGENGSAGEPGPKGERGLS
                     GPPGLPGIPGPAGKEGPSGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSTGAKGSTGP
                     KGERGAPGVQGAPGNAGAAGPAGPAGPQGAPGSRGPPGLKGDRGVPGDRGIKGESGLPD
                     SAALRQQMEALKGKLQRLEVAFSHYQKAALFPDGRTEPRPALTITTSPNLGTRENNADQ
                     VTPVSHIGCPNTTQQGSPVFAKLLAKNQASLCNTTLNWHSQDGAGSSYLSQGLRYEEDK
                     KELVVDSPGLYYVFLELKLSPTFTNTGHKVQGWVSLVLQAKPQVDDFDNLALTVELFPC
                     SMENKLVDRSWSQLLLLKAGHRLSVGLRAYLHGAQDAYRDWELSYPNTTSFGLFLVKPD
                     NPWE"
     regulatory      complement(3176..3398)
                     /regulatory_class="polyA_signal_sequence"
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3179..3386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      complement(3399..3433)
                     /regulatory_class="terminator"
                     /note="lambda To terminator"
     terminator      3402..3436
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3454..4248)
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /protein_id="ACT68290.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      4660..5248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"