LOCUS       Exported                8361 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRama-34, complete sequence.
ACCESSION   GQ258349
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Ganguly S, Liu J, Pillai VB, Mittler RS, Amara RR.
  TITLE     Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a 
            HIV-1 Gag DNA vaccine: different effects on cellular and humoral 
            immunity
  JOURNAL   Vaccine 28 (5), 1300-1309 (2010)
  PUBMED    19944789
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Ganguly S, Amara RR.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2009) Microbiology and Immunology, Emory 
            University, 954 Gatewood Road NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 8361)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Cloning vector pRama-34"
                     /mol_type="other DNA"
                     /db_xref="taxon:658872"
     regulatory      1..690
                     /regulatory_class="promoter"
                     /note="CMV immediate early promoter"
     enhancer        93..474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          691..1638
                     /number=A
                     /note="CMV intron A"
     intron          814..1640
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             1650..3185
                     /codon_start=1
                     /product="clade B consensus optimized HIV-1 Gag"
                     /label=clade B consensus optimized HIV-1 Gag
                     /protein_id="ACT68286.1"
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSSQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPA
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNTATIMMQRGNFRNQRKMVKSFNSGKEG
                     HTARNCRAPRKKGSWKSGKEGHQMKDCTERQANFLGKIWPSYKGRPGNFLQSRPEPTAP
                     PFLQSRPEPTAPPEESFRSGVETTTPPQKQEPIDKELYPLTSLRSLFGNDPSS"
     regulatory      complement(3222..3444)
                     /regulatory_class="polyA_signal_sequence"
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3225..3432
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      complement(3445..3479)
                     /regulatory_class="terminator"
                     /note="Lambda To terminator"
     terminator      3448..3481
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     regulatory      3534..4223
                     /regulatory_class="promoter"
                     /note="CMV immediate early promoter"
     enhancer        3626..4007
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4008..4211
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          4224..5171
                     /number=A
                     /note="CMV intron A"
     intron          4347..5173
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5292..6221
                     /codon_start=1
                     /product="4-1BBL"
                     /label=4-1BBL
                     /note="co-stimulatory molecule, CD137 ligand"
                     /protein_id="ACT68287.1"
                     /translation="MDQHTLDVEDTADARHPAGTSCPSDAALLRDTGLLADAALLSDTV
                     RPTNAALPTDAAYPAVNVRDREAAWPPALNFCSRHPKLYGLVALVLLLLIAACVPIFTR
                     TEPRPALTITTSPNLGTRENNADQVTPVSHIGCPNTTQQGSPVFAKLLAKNQASLCNTT
                     LNWHSQDGAGSSYLSQGLRYEEDKKELVVDSPGLYYVFLELKLSPTFTNTGHKVQGWVS
                     LVLQAKPQVDDFDNLALTVELFPCSMENKLVDRSWSQLLLLKAGHRLSVGLRAYLHGAQ
                     DAYRDWELSYPNTTSFGLFLVKPDNPWE"
     regulatory      6225..6447
                     /regulatory_class="polyA_signal_sequence"
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    6228..6435
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      6448..6482
                     /regulatory_class="terminator"
                     /note="lambda To terminator"
     terminator      6451..6485
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(6503..7297)
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /protein_id="ACT68288.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      7709..8297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"