LOCUS       Exported                4245 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  Cloning vector pRAF800, complete sequence.
ACCESSION   AY091640
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Boucher I, Parrot M, Gaudreau H, Champagne CP, Vadeboncoeur C, 
            Moineau S.
  TITLE     Novel food-grade plasmid vector based on melibiose fermentation for 
            the genetic engineering of Lactococcus lactis
  JOURNAL   Appl. Environ. Microbiol. 68 (12), 6152-6161 (2002)
  PUBMED    12450840
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Boucher I, Parrot M, Vadeboncoeur C, Moineau S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2002) Biochimie et Microbiologie, Universite 
            Laval, Quebec G1K 7P4, Canada
REFERENCE   3  (bases 1 to 4245)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Cloning vector pRAF800"
                     /mol_type="genomic DNA"
                     /note="food-grade cloning vector based on Lactococcus 
                     lactis plasmid pSRQ800 replicon and melibiose fermentation 
                     conferred by Lactococcus raffinolactis ATCC 43920 
                     alpha-galactosidase"
                     /db_xref="taxon:192755"
     gene            186..2393
                     /gene="aga"
                     /label=aga
     CDS             186..2393
                     /codon_start=1
                     /gene="aga"
                     /product="alpha-galactosidase Aga"
                     /label=aga
                     /note="derived from Lactococcus raffinolactis ATCC 43920"
                     /protein_id="AAM18789.1"
                     /translation="MTLITFDENNKIFHLSNTSISYLIGIEKESYLSHLYFGKVIKTYH
                     AGRKYPAMNRSFSPNPDGMPLNTRDFSLDVISQEFPSYGHGDFRNPAVQIKQTNGSSIT
                     EFVYDSYEIISGKPILDGLPATYVGGDEEAETLVITLIDKLLNLKLKLSYTIYAQRNVI
                     ARNALLENNGMAPVVIEKLASLSVDLPEQDLELISLPGRHVKEREIERQTIQRGTRIID
                     SKRGASSHQSNPFIAIVEPKTDEFTGTAIGLTLVYSGNHEMLVERDQFSQTRVMAGINP
                     FGFEWELESDASFQSPEALLVYSDQGLNGMSQTFHDLLQNRLARGQYRQAERPILINNW
                     EATYFDFDTDKIKKIVDSAADLGIELFVLDDGWFGKRDDDTSGLGDWFENTEKLKGGLK
                     GIADYVHQKNMTFGLWFEPEMVNADSDLFRQHPDYALQIPGRSMSTSRDQYVLDFSRKE
                     VRETITAQMRAILDTIDIDYIKWDMNRNLTEVYSATASAAHQGEVFHRYVLGLYEMLEE
                     LTTDYDHILWEGCSGGGGRFDAGFLHYMPQSWTSDNTDAVERLDIQYGTSLVYPISSMG
                     AHVSAVPNHQTYRETGLEIRGDVAMSGVFGYELNLQDMTQEEKAVVLEQVAFYKTHRKL
                     LQYGKFHRLLSPFESDQTAWLFVNGDQSQAIGFYFRKYAESAGPLRTLKFTGLAPEKTY
                     QVNGDAIYGGDELMSVGLHIYPFLVGDYQSRKFVINEVKSN"
     gene            complement(2523..3728)
                     /gene="repB"
                     /label=repB
     CDS             complement(2523..3728)
                     /codon_start=1
                     /gene="repB"
                     /product="plasmid replication initiator RepB"
                     /label=repB
                     /note="derived from Lactococcus lactis plasmid pSRQ800"
                     /protein_id="AAM18790.1"
                     /translation="MSIIPKNNEEKKQKVVCTLNDLEKRKVVEHNALIQSVAKMQKTAL
                     KMFELAVSCIDTDKIPENNTVYLSKKELFSFFEVSSSSKHSQFKEAIEFMQKQAYFKIK
                     SNKALGIEYESIVPIPYVKWNDYNDEVLIRFSPEIMPYLINLKAEFTQYKISELQKLNS
                     KYSIILYRWLSMSYNQYEHYSNKGGRRAEQVEAYQNPSITVKELRAITDTVNEYKHISN
                     FFKRIIEEPLEEINAHTSFNVTYDKIKKGRSIDSIVFHIEKKRMADDNSYKLDNRAYQE
                     DKKQKETDNRLLAGEALENKYTKLLIEHFLLSPYDMTDTATMAGLQKNVYPLYDELKDL
                     RGLNGVKDHLSYVASKKEAYSKRNVAKYLKKAIEQYLPTVKLQDLEQPERAKVRGKGAS
                     HE"
     rep_origin      complement(3728..4245)
                     /direction=LEFT
                     /label=derived from Lactococcus lactis plasmid pSRQ800
                     /note="derived from Lactococcus lactis plasmid pSRQ800"