LOCUS       Exported                4635 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pRADM, complete sequence.
ACCESSION   JN596961
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Yao L, Sun J.
  TITLE     Construction of a baculovirus-silkworm multigene expression system 
            and its application on producing virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Yao L, Sun J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu 
            Mountain Insect Biology, Nan Yang Normal University, 1638 Wolong 
            Road, Nan Yang, He Nan 473061, China
REFERENCE   3  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Baculovirus expression vector pRADM"
                     /mol_type="other DNA"
                     /db_xref="taxon:1129738"
     promoter        70..174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             175..1035
                     /codon_start=1
                     /gene="ampR"
                     /product="ampicillin resistance protein"
                     /label=ampR
                     /protein_id="AEY64193.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            175..1035
                     /gene="ampR"
                     /label=ampR
     oriT            1229..1519
                     /note="origin of transfer for the bacterial F plasmid 
                     (Frost et al., 1994)"
     rep_origin      1569..1925
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     mobile_element  2054..2219
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     regulatory      complement(2248..2487)
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 polyA"
     polyA_signal    2248..2382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2605..2726)
                     /regulatory_class="promoter"
                     /note="polh"
     promoter        complement(2635..2726)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     regulatory      2766..2886
                     /regulatory_class="promoter"
                     /note="p10"
     promoter        2771..2880
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      2951..3232
                     /regulatory_class="polyA_signal_sequence"
                     /note="HSV tk polyA"
     polyA_signal    3016..3064
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3581..3609
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3798..4331
                     /codon_start=1
                     /gene="GmR"
                     /product="gentamycin resistance protein"
                     /label=GmR
                     /protein_id="AEY64194.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            3798..4331
                     /gene="GmR"
                     /label=GmR
     mobile_element  4398..4622
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"