LOCUS       Exported                6447 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRAB11, complete sequence.
ACCESSION   JN635500
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Helle L, Kull M, Mayer S, Marincola G, Zelder ME, Goerke C, Wolz C, 
            Bertram R.
  TITLE     Vectors for improved Tet repressor-dependent gradual gene induction 
            or silencing in Staphylococcus aureus
  JOURNAL   Microbiology (Reading, Engl.) 157 (PT 12), 3314-3323 (2011)
  PUBMED    21921101
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Bertram R.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2011) Dept of Microbial Genetics, University of 
            Tuebingen, Waldhaeuser Str. 70/8, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 6447)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Cloning vector pRAB11"
                     /strain="SA113"
                     /mol_type="other DNA"
                     /label=derived from Staphylococcus aureus
                     /note="derived from Staphylococcus aureus"
                     /db_xref="taxon:1096516"
     protein_bind    244..265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        280..310
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    318..334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     342..358
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(901..1551)
                     /codon_start=1
                     /gene="cat"
                     /product="CAT"
                     /label=cat
                     /note="chloramphenicol acetyl transferase"
                     /protein_id="AEQ63302.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(901..1551)
                     /gene="cat"
                     /label=cat
     rep_origin      2009..2944
     CDS             3042..3056
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(3368..3994)
                     /codon_start=1
                     /product="TetR(B)"
                     /label=TetR
                     /note="tetracycline repressor class (B)"
                     /protein_id="AEQ63303.1"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             complement(3368..3991)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     regulatory      complement(4010..4065)
                     /regulatory_class="promoter"
                     /note="pR* w/o tetO"
     regulatory      4067..4151
                     /regulatory_class="promoter"
                     /note="Pxyl/tet"
     protein_bind    4101..4119
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    4136..4153
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(4189..4205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4679..4783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4784..5644
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AEQ63304.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5815..6403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5896..6447