LOCUS       Exported                5955 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQS-SAH, complete sequence.
ACCESSION   KP294374
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Quorum-sensing Salmonella selectively trigger protein expression 
            within tumors
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (11), 3457-3462 (2015)
  PUBMED    25737556
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2014) Chemical Engineering, University of 
            Massachusetts Amherst, 686 North Pleasant Street, Amherst, MA 
            01003-9303, USA
REFERENCE   3  (bases 1 to 5955)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Cloning vector pQS-SAH"
                     /mol_type="other DNA"
                     /db_xref="taxon:1628428"
     rep_origin      20..690
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             892..2067
                     /codon_start=1
                     /product="aspartate semialdehyde dehydrogenase"
                     /label=aspartate semialdehyde dehydrogenase
                     /note="ASD"
                     /protein_id="AJT39556.1"
                     /translation="MVKDAPQDTGAHTQHISLQEKNAMKNVGFIGWRGMVGSVLMQRMV
                     EERDFDAIRPVFFSTSQFGQAAPTFGDTSTGTLQDAFDLDALKALDIIVTCQGGDYTNE
                     IYPKLRESGWQGYWIDAASTLRMKDDAIIILDPVNQDVITDGLNNGVKTFVGGNCTVSL
                     MLMSLGGLFAHNLVDWVSVATYQAASGGGARHMRELLTQMGQLYGHVADELATPSSAIL
                     DIERKVTALTRSGELPVDNFGVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTAS
                     VIPVDGLCVRVGALRCHSQAFTIKLKKEVSIPTVEELLAAHNPWAKVVPNDRDITMREL
                     TPAAVTGTLTTPVGRLRKLNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     terminator      2225..2319
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2340..2999)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=chloramphenicol acetyltransferase
                     /note="Cm(r)"
                     /protein_id="AJT39553.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(3000..3102)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     regulatory      3132..3276
                     /regulatory_class="promoter"
                     /note="luxI"
     CDS             3312..4271
                     /codon_start=1
                     /product="alpha-hemolysin"
                     /label=alpha-hemolysin
                     /note="SAH; derived from Staphylococcus aureus"
                     /protein_id="AJT39557.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNTT
                     VKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKSGL
                     AWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIG
                     ANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLF
                     MKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYERVRDD
                     YQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
     RBS             4351..4362
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4370..5122
                     /codon_start=1
                     /product="regulatory protein LuxR"
                     /label=regulatory protein LuxR
                     /note="LuxR"
                     /protein_id="AJT39554.1"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK
                     KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP
                     SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ
                     MKLNTTNRCQSISKAILTGAIDCPYFKN"
     CDS             5153..5734
                     /codon_start=1
                     /product="autoinducer synthase LuxI"
                     /label=autoinducer synthase LuxI
                     /note="LuxI"
                     /protein_id="AJT39555.1"
                     /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES
                     DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR
                     FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI
                     GDKEIHVLGDTKSVVLSMPINEQFKKAVLN"