LOCUS       Exported                4823 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQLinkN, complete sequence.
ACCESSION   EF196676
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K.
  TITLE     Vectors for co-expression of an unrestricted number of proteins
  JOURNAL   Nucleic Acids Res. 35 (6), E43 (2007)
  PUBMED    17311810
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Kuemmel D, Scheich C, Buessow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2006) Vertebrate Genomics, Max Planck Institute 
            for Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 4823)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Cloning vector pQLinkN"
                     /mol_type="other DNA"
                     /db_xref="taxon:420414"
     misc_feature    7..27
                     /label=LIC cassette with PacI restriction site
                     /note="LIC cassette with PacI restriction site"
     promoter        36..80
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    56..72
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      56..61
                     /regulatory_class="minus_35_signal"
     regulatory      74..79
                     /regulatory_class="minus_10_signal"
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      127..133
                     /regulatory_class="ribosome_binding_site"
     misc_feature    141
                     /note="translation start site for inserts cloned into the 
                     multiple cloning site"
     misc_feature    144..214
                     /note="multiple cloning site; includes BamHI, NotI and 
                     HindIII restriction sites"
     protein_bind    complement(184..208)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      230..324
                     /regulatory_class="terminator"
                     /note="lambda t0 transcription terminator"
     terminator      230..324
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    329..378
                     /label=LIC cassette with SwaI, PacI restriction sites
                     /note="LIC cassette with SwaI, PacI restriction sites"
     CDS             418..1077
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     regulatory      1136..1233
                     /regulatory_class="terminator"
                     /note="rrnB T1 transcription terminator"
     terminator      1142..1228
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1321..2403)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="overproducing allele Q; similar to lac repressor 
                     encoded by GenBank Accession Number U78872"
                     /protein_id="ABM91082.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     gene            complement(1321..2403)
                     /gene="lacI"
                     /label=lacI
     promoter        complement(2404..2481)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    2673..2813
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      2997
                     /label=ColE1 ori
                     /note="ColE1 ori"
     rep_origin      complement(2999..3587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3758..4618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase precursor"
                     /label=bla
                     /note="penicillinase; similar to the protein encoded by 
                     GenBank Accession Number AF074376"
                     /protein_id="ABM91081.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3758..4618)
                     /gene="bla"
                     /label=bla
     promoter        complement(4619..4723)
                     /gene="bla"
                     /label=AmpR promoter