LOCUS       Exported                7015 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQC2319, complete sequence.
ACCESSION   MH587015
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson PE Jr., Hinton DM, Stibitz S.
  TITLE     A novel Bvg-repressed promoter causes vrg-like transcription of fim3
            but does not result in the production of serotype 3 Fimbriae in the 
            Bvg(-) mode Bordetella pertussis
  JOURNAL   J. Bacteriol. (2018) In press
  PUBMED    30061354
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Chen Q, Lee G, Craig C, Ng V, Carlson P, Hinton D, Stibitz S.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2018) Division of Bacterial, Parasitic, and 
            Allergenic Products, Center for Biologics Evaluation and Research, 
            FDA, 10903 New Hampshire Avenue, Silver Spring, MD 20933, USA
REFERENCE   3  (bases 1 to 7015)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Cloning vector pQC2319"
                     /mol_type="other DNA"
                     /db_xref="taxon:2293732"
     regulatory      58..144
                     /regulatory_class="terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene; from the Photorhabdus luminescens bioluminescence 
                     operon"
     terminator      58..144
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(221..934)
                     /codon_start=1
                     /gene="RFP"
                     /product="RFP"
                     /label=RFP
                     /protein_id="AXK90409.1"
                     /translation="MRGSGSMSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQ
                     TMKIKVVEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYED
                     GGVLTATQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGH
                     SQMALKLVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEM
                     AVAKYCDLPSKLGHR"
     gene            complement(221..934)
                     /gene="RFP"
                     /label=RFP
     regulatory      1251..1337
                     /regulatory_class="terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1251..1337
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      1434..1520
                     /regulatory_class="terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1434..1520
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2288..2316
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2505..3038
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=aacC1
                     /note="confers resistance to gentamycin"
                     /protein_id="AXK90410.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            2505..3038
                     /gene="aacC1"
                     /label=aacC1
     oriT            3057..3165
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
     CDS             4587..4778
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label=rop
                     /note="maintains plasmids at low copy number"
                     /protein_id="AXK90411.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     gene            4587..4778
                     /gene="rop"
                     /label=rop
     misc_feature    4880..5020
                     /note="basis of mobility region from pBR322; bom"
     rep_origin      complement(5206..5794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5965..6825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /protein_id="AXK90412.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5965..6825)
                     /gene="bla"
                     /label=bla
     promoter        complement(6826..6930)
                     /gene="bla"
                     /label=AmpR promoter