LOCUS       Exported               10973 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  P-element cloning system vector pP{wlo-hsinGS}, complete sequence.
ACCESSION   AF524837
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Roman G, Endo K, Zong L, Davis RL.
  TITLE     P[Switch], a system for spatial and temporal control of gene 
            expression in Drosophila melanogaster
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 98 (22), 12602-12607 (2001)
  PUBMED    11675496
REFERENCE   2  (bases 1 to 10973)
  AUTHORS   Roman G, Davis RL.
  TITLE     Conditional expression of UAS-transgenes in the adult eye with a new
            gene-switch vector system
  JOURNAL   Genesis 34 (1-2), 127-131 (2002)
  PUBMED    12324966
REFERENCE   3  (bases 1 to 10973)
  AUTHORS   Roman G, Davis RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2002) Molecular and Cellular Biology, Baylor 
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   4  (bases 1 to 10973)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="P-element cloning system vector pP{wlo-hsinGS}"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:201840"
     misc_feature    1..586
                     /label=P element 5 end
                     /note="P element 5 end"
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="mini-white"
                     /product="white"
                     /label=mini-white
                     /protein_id="AAM82584.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     gene            complement(1364..4246)
                     /gene="mini-white"
                     /label=mini-white
     protein_bind    complement(4738..4771)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     complement(4738..4762)
                     /label=loxP
                     /note="loxP"
     promoter        4782..5019
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    4784..5039
                     /label=HSP70Bb promoter
                     /note="HSP70Bb promoter"
     intron          5053..5110
                     /note="from P transposase first intron"
     CDS             5121..7091
                     /codon_start=1
                     /gene="Gene-Switch"
                     /product="Gene-Switch"
                     /label=Gene-Switch
                     /note="transcription factor; Gal4-hPR-p65 fusion protein"
                     /protein_id="AAM82585.1"
                     /translation="MDSQQPDLKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWEC
                     RYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLEFPG
                     VDQKKFNKVRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIPPLINLLMSIE
                     PDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYS
                     WMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSFYSLCLTMWQIPQEFVKLQV
                     SQEEFLCMKVLLLLNTIPLEGLRSQTQFEEMRSSYIRELIKAIGLRQKGVVSSSQRFYQ
                     LTKLLDNLHDLVKQLHLYCLNTFIQSRALSVEFPEMMSEVIAGSTRYQAEFQYLPDTDD
                     RHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTS
                     SLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPV
                     LAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDN
                     SEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSG
                     DEDFSSIADMDFSALLSQISS"
     gene            5121..7091
                     /gene="Gene-Switch"
                     /label=Gene-Switch
     CDS             6717..7088
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (OShea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    7700..7938
                     /label=HSP70 termination sequence
                     /note="HSP70 termination sequence"
     primer_bind     complement(7940..7956)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7981..8213
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    8214..8714
                     /label=white linker sequence
                     /note="white linker sequence"
     misc_feature    8715..10744
                     /label=pUC8 plasmid sequence
                     /note="pUC8 plasmid sequence"
     promoter        9015..9119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9120..9980
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10151..10739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    10745..10973
                     /label=white linker sequence
                     /note="white linker sequence"