LOCUS       Exported                7237 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Targeting vector pP{white-STAR}, complete sequence.
ACCESSION   FJ710212
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Choi CM, Vilain S, Langen M, Van Kelst S, De Geest N, Yan J, 
            Verstreken P, Hassan BA.
  TITLE     Conditional mutagenesis in Drosophila
  JOURNAL   Science 324 (5923), 54 (2009)
  PUBMED    19342580
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Choi CM, Vilain S, Langen M, Van Kelst S, De Geest N, Yan J, 
            Verstreken P, Hassan BA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2009) Department of Molecular and Developmental 
            Genetics, VIB, Herestraat 49, Leuven 3000, Belgium
REFERENCE   3  (bases 1 to 7237)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Targeting vector pP{white-STAR}"
                     /mol_type="other DNA"
                     /label=fly transformation vector
                     /note="fly transformation vector"
                     /db_xref="taxon:619306"
     misc_feature    25..42
                     /label=I-SceI site
                     /note="I-SceI site"
     protein_bind    43..76
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    43..55
                     /label=FRT
                     /note="FRT"
     misc_feature    102..687
                     /label=P element 5 end
     misc_feature    104..678
                     /label=P element 5 end
                     /note="P element 5 end"
     misc_feature    complement(688..2677)
                     /label=pUC backbone sequence
                     /note="pUC backbone sequence"
     rep_origin      complement(922..1510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1681..2541)
                     /codon_start=1
                     /gene="amp"
                     /product="amp"
                     /label=amp
                     /note="ampicilin resistance protein"
                     /protein_id="ACN64943.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1681..2541)
                     /gene="amp"
                     /label=amp
     promoter        complement(2542..2646)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    complement(2731..2964)
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    2731..2963
                     /label=P element 3 end
                     /note="P element 3 end"
     protein_bind    3026..3059
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    3026..3038
                     /label=FRT
                     /note="FRT"
     misc_feature    3060..3077
                     /label=I-SceI site
                     /note="I-SceI site"
     misc_feature    3104..3153
                     /label=attP
                     /note="attP"
     CDS             join(3646..3717,4113..4385,4460..5110,5176..5490,5714..5843,
                     5914..6527)
                     /codon_start=1
                     /gene="white"
                     /product="white"
                     /label=white
                     /protein_id="ACN64944.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNVTADASQSCINQGFGQAKNYG
                     TLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERHI
                     PAPRKHLLKNGCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNGQ
                     PVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQELS
                     LSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQV
                     LKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSWVPSVLPT
                     TIRRTFTYRCWPLCPDGRSSPVIGSPRYGDNFAISKVARDMEQLLATKNLEKPLEQPEN
                     GYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTVAILIGLIFLGQQLTQVGVMN
                     INGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPLFLT
                     VPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSVGPP
                     VIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSSNTT
                     CPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     gene            3646..6527
                     /gene="white"
                     /label=white
     misc_feature    complement(7186..7235)
                     /label=attP
                     /note="attP"