LOCUS       Exported               10691 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  P-element cloning system vector pP{Switch2}, complete sequence.
ACCESSION   AF524839
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Roman G, Endo K, Zong L, Davis RL.
  TITLE     P[Switch], a system for spatial and temporal control of gene 
            expression in Drosophila melanogaster
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 98 (22), 12602-12607 (2001)
  PUBMED    11675496
REFERENCE   2  (bases 1 to 10691)
  AUTHORS   Roman G, Davis RL.
  TITLE     Conditional Expression of UAS-Transgenes in the Adult Eye with a New
            Gene-Switch Vector System
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10691)
  AUTHORS   Roman G, Davis RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2002) Molecular and Cellular Biology, Baylor 
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   4  (bases 1 to 10691)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="P-element cloning system vector pP{Switch2}"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:201842"
     misc_feature    1..586
                     /label=P element 5 end
     misc_feature    1..585
                     /label=P element 5 end
                     /note="P element 5 end"
     CDS             join(153..442,501..2565)
                     /codon_start=1
                     /product="Gene-Switch"
                     /label=Gene-Switch
                     /note="transcription factor; P transposase-Gal4-hPR-p65 
                     fusion protein"
                     /protein_id="AAM82587.1"
                     /translation="MKYCKFCCKAVTGVKLIHVPKCAIKRKLWEQSLGCSLGENSQICD
                     THFNDSQWKAAPAKGQTFKRRRLNADAVPSKVIEPEPEKIKEGYTSGSTQTESCSLFNE
                     NKSLREKIRTLEYEMRRLEQQPNSMDSQQPDLKLLSSIEQACDICRLKKLKCSKEKPKC
                     AKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQ
                     DIKALLEFPGVDQKKFNKVRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIP
                     PLINLLMSIEPDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHI
                     DDQITLIQYSWMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSFYSLCLTMWQ
                     IPQEFVKLQVSQEEFLCMKVLLLLNTIPLEGLRSQTQFEEMRSSYIRELIKAIGLRQKG
                     VVSSSQRFYQLTKLLDNLHDLVKQLHLYCLNTFIQSRALSVEFPEMMSEVIAGSTRYQA
                     EFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPK
                     PAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSA
                     LAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPA
                     VFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGA
                     PGLPNGLLSGDEDFSSIADMDFSALLSQISS"
     intron          443..500
                     /note="from P transposase first intron"
     CDS             2191..2562
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (OShea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    3174..3412
                     /label=HSP70 termination sequence
                     /note="HSP70 termination sequence"
     misc_feature    complement(3419..7665)
                     /label=mini-white selectable marker
                     /note="mini-white selectable marker"
     misc_feature    7698..7931
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    7699..7931
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    7932..8432
                     /label=white linker sequence
                     /note="white linker sequence"
     misc_feature    8433..10462
                     /label=pUC8 plasmid sequence
                     /note="pUC8 plasmid sequence"
     promoter        8733..8837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8838..9698
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9869..10457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    10463..10691
                     /label=white linker sequence
                     /note="white linker sequence"