LOCUS       Exported               10941 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  P-element cloning system vector pP{Switch1}, complete sequence.
ACCESSION   AF524838
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Roman G, Endo K, Zong L, Davis RL.
  TITLE     P[Switch], a system for spatial and temporal control of gene 
            expression in Drosophila melanogaster
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 98 (22), 12602-12607 (2001)
  PUBMED    11675496
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   Roman G, Davis RL.
  TITLE     Conditional Expression of UAS-Transgenes in the Adult Eye with a New
            Gene-Switch Vector System
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10941)
  AUTHORS   Roman G, Davis RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2002) Molecular and Cellular Biology, Baylor 
            College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
REFERENCE   4  (bases 1 to 10941)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="P-element cloning system vector pP{Switch1}"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:201841"
     misc_feature    1..147
                     /label=P element 5 end
                     /note="P element 5 end"
     CDS             335..2305
                     /codon_start=1
                     /gene="Gene-Switch"
                     /product="Gene-Switch"
                     /label=Gene-Switch
                     /note="transcription factor; Gal4-hPR-p65 fusion protein"
                     /protein_id="AAM82586.1"
                     /translation="MDSQQPDLKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWEC
                     RYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLEFPG
                     VDQKKFNKVRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIPPLINLLMSIE
                     PDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYS
                     WMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSFYSLCLTMWQIPQEFVKLQV
                     SQEEFLCMKVLLLLNTIPLEGLRSQTQFEEMRSSYIRELIKAIGLRQKGVVSSSQRFYQ
                     LTKLLDNLHDLVKQLHLYCLNTFIQSRALSVEFPEMMSEVIAGSTRYQAEFQYLPDTDD
                     RHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTS
                     SLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPV
                     LAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDN
                     SEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSG
                     DEDFSSIADMDFSALLSQISS"
     gene            335..2305
                     /gene="Gene-Switch"
                     /label=Gene-Switch
     CDS             1931..2302
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (OShea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    2914..3152
                     /label=HSP70 termination sequence
                     /note="HSP70 termination sequence"
     primer_bind     complement(3154..3170)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     3196..3229
                     /label=loxP
                     /note="loxP"
     protein_bind    3196..3229
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(3248..3264)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3273..7499
                     /label=mini-white selectable marker
                     /note="mini-white selectable marker"
     misc_feature    7735..10586
                     /label=pBluescriptII plasmid sequence
                     /note="pBluescriptII plasmid sequence"
     promoter        complement(7748..7766)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7776..7792)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7933..8388)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8415..8519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8520..9380
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9551..10139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    10427..10448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10463..10493
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10501..10517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10525..10541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10562..10580
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_recomb     10599..10632
                     /label=loxP
                     /note="loxP"
     protein_bind    10599..10632
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    10705..10941
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    10709..10941
                     /label=P element 3 end
                     /note="P element 3 end"