LOCUS       Exported                5638 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPZP500, complete sequence.
ACCESSION   JX025644
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     pMAA-Red: Binary cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2012) Department of Crop Sciences, Division of 
            Plant Protection, University of Natural Resources and Life Sciences,
            Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria
REFERENCE   3  (bases 1 to 5638)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Cloning vector pPZP500"
                     /mol_type="other DNA"
                     /db_xref="taxon:1200951"
     CDS             404..1033
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1462..2535
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QATADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2601..2795
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(2893..3532)
                     /direction=LEFT
                     /note="bom region; derived from pBR322"
     rep_origin      complement(3148..3736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3980..4771)
                     /codon_start=1
                     /gene="aadA1"
                     /product="streptomycin adenylyltransferase"
                     /function="confers streptomycin and spectinomycin 
                     resistance"
                     /label=aadA1
                     /protein_id="AFM74427.1"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(3980..4771)
                     /gene="aadA1"
                     /label=aadA1
     misc_feature    5056..5310
                     /label=left border T-DNA repeat
                     /note="left border T-DNA repeat"
     misc_feature    5294..5318
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    5311..5438
                     /note="polylinker; multiple cloning site"
     misc_feature    5387..5443
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    5439..5638
                     /label=right border T-DNA repeat
                     /note="right border T-DNA repeat"
     misc_feature    5500..5524
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"