LOCUS       Exported                4769 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pPyr-Cm, complete sequence.
ACCESSION   AY464558
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus 
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
  PUBMED    15109830
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of 
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 4769)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Integration vector pPyr-Cm"
                     /lab_host="Bacillus subtilis"
                     /mol_type="other DNA"
                     /note="origin of replication and ampicillin resistance for 
                     use in Escherichia coli, integrates into the chromosome of 
                     Bacillus subtilis at the pyrD locus; vector pieced together
                     from pUC18, pDG364, and parts of the pyrD gene of Bacillus 
                     subtilis"
                     /db_xref="taxon:257525"
     misc_feature    complement(54..495)
                     /note="3 end of dihydroorotate dehydrogenase (catalytic 
                     subunit) (pyrD) of Bacillus subtilis"
     CDS             complement(904..1554)
                     /codon_start=1
                     /gene="CAT"
                     /product="chloramphenicol acetyltransferase"
                     /label=CAT
                     /note="chloramphenicol resistance"
                     /protein_id="AAR30044.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(904..1554)
                     /gene="CAT"
                     /label=CAT
     misc_feature    complement(2039..2458)
                     /note="5 end of dihydroorotate dehydrogenase (pyrD) of 
                     Bacillus subtilis"
     promoter        2863..2967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2968..3828
                     /codon_start=1
                     /gene="BLA"
                     /product="beta-lactamase"
                     /label=BLA
                     /note="ampicillin resistance"
                     /protein_id="AAR30045.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2968..3828
                     /gene="BLA"
                     /label=BLA
     rep_origin      3999..4587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"