LOCUS       Exported                9139 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPYPX99, complete sequence.
ACCESSION   AY178634
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Yao Q, Peng R, Xiong A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2002) Shanghai Academy of Agricultural Sciences, 
            Agro-Biotechnology Research Center, 2901 Beidi Rd, Shanghai 201106, 
            China
REFERENCE   2  (bases 1 to 9139)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     NCBI staff are still waiting for submitters to provide appropriate 
            coding region information.
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Expression vector pPYPX99"
                     /lab_host="Pichia pastoris"
                     /mol_type="other DNA"
                     /db_xref="taxon:218468"
     misc_feature    1..936
                     /label=modified AOX1 promoter
                     /note="modified AOX1 promoter"
     misc_feature    937..1212
                     /label=similar to modified alpha factor secretion signal
                     /note="similar to modified alpha factor secretion signal"
     regulatory      1275..1607
                     /regulatory_class="terminator"
                     /note="AOX1 transcription terminator"
     terminator      1343..1589
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     misc_feature    complement(1994..4527)
                     /label=similar to phosphoribosyl-ATP pyrophosphohydrolase
                     /note="similar to phosphoribosyl-ATP pyrophosphohydrolase"
     CDS             4798..5613
                     /codon_start=1
                     /product="aminoglycoside-3-O-phosphotransferase"
                     /label=aminoglycoside-3-O-phosphotransferase
                     /protein_id="AAO84033.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    5985..6741
                     /label=AOX1 3 fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    5987..6736
                     /label=3 AOX1 fragment from Pichia pastoris
                     /note="3 AOX1 fragment from Pichia pastoris"
     misc_feature    6884..7024
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7151..7824)
                     /direction=LEFT
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(7210..7798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7969..8829)
                     /codon_start=1
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=beta-lactamase
                     /protein_id="AAO84034.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8830..8934)
                     /gene="bla"
                     /label=AmpR promoter