LOCUS       Exported                9219 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPW380, complete sequence.
ACCESSION   DQ164215
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Woo PC, Ma SS, Teng JL, Li MW, Kao RY, Lau SK, Yuen KY.
  TITLE     Construction of an inducible expression shuttle vector for 
            Laribacter hongkongensis, a novel bacterium associated with 
            gastroenteritis
  JOURNAL   FEMS Microbiol. Lett. 252 (1), 57-65 (2005)
  PUBMED    16165318
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   Woo PCY., Ma SSL., Teng JLL., Li MWS., Lau SKP., Yuen KY.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2005) Microbiology, The University of Hong Kong, 
            Hong Kong
REFERENCE   3  (bases 1 to 9219)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Shuttle vector pPW380"
                     /lab_host="Laribacter hongkongensis HLHK8"
                     /mol_type="other DNA"
                     /db_xref="taxon:342567"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    469..590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     952..968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        975..993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1981..2604
                     /codon_start=1
                     /gene="parA"
                     /product="ATPase"
                     /label=parA
                     /note="involved in chromosome partitioning"
                     /protein_id="AAZ91679.1"
                     /translation="MRTIVVASQKGGVGKTTIAGHLGVMAEQSKEGPVALIDTDPQGSL
                     ASWWNERTDEAPLFARVEINKLTEHLQALAKGGIKLAIIDTPPSVTEMIQQVLRTADLV
                     LIPTRPSPHDLRAVGSTVELVENAGKRMIFVINGAAPRARIAGEAAVALSQHGTVAPVT
                     LYQRTDFASSMIDGRTVQEIDPKGRSAEEIGKLWKYVSTQLRKI"
     gene            1981..2604
                     /gene="parA"
                     /label=parA
     CDS             3058..3975
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label=repA
                     /note="plasmid replication initiator protein"
                     /protein_id="AAZ91680.1"
                     /translation="MAEVVDEQPARRKRTPKGPRGLMPVHHPNRDFFLCDLFNYALKDD
                     GVSMEAPIFTLATKPDTSVWHWESKDGARAITVTPSVKGRATQFDKDLLIYVVSQMTEA
                     MNRGRPDANSRTVRFRVYDYLVSTNKPTGGKEYQRLEDALDRLRGTSIKTNIKTGGQRV
                     KEGFGIVDSWTIIEKAPDDDRMIAVEVTLSKWLFNAVQAHEVLTINPDYFRLRKPIERR
                     LYELARKHCGDQAFFVIGLELLQDKCGSKSALFEFRRALREIIKADTLPDYRMTLDNEK
                     DQVIFYTRDAKKLTASAALPQRFQ"
     gene            3058..3975
                     /gene="repA"
                     /label=repA
     promoter        complement(5841..5859)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5880..5896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5904..5920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6029..6232)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6233..6536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6711..7299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    7628..7675
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7907..8701)
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(8736..9093)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8750..8885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9095..9199)
                     /gene="bla"
                     /label=AmpR promoter