LOCUS       Exported                4168 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPTbsr, complete sequence.
ACCESSION   MH541819
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Buck JM.
  TITLE     Blasticidin-S deaminase, a new selection marker for genetic 
            transformation of the diatom Phaeodactylum tricornutum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Buck JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2018) Department of Biology, University of 
            Konstanz, Universitatsstrasse 10, Konstanz 78457, Germany
REFERENCE   3  (bases 1 to 4168)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Cloning vector pPTbsr"
                     /mol_type="other DNA"
                     /db_xref="taxon:2301586"
     CDS             1105..1527
                     /codon_start=1
                     /gene="bsr"
                     /product="blasticidin-S deaminase"
                     /label=bsr
                     /protein_id="AXN72345.1"
                     /translation="MKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIIS
                     AVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCR
                     ELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     gene            1105..1527
                     /gene="bsr"
                     /label=bsr
     promoter        complement(1792..1810)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2068..2656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2827..3687)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3688..3792)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4138..4156
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"