LOCUS       Exported                6218 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pPTB-HcRED, complete sequence.
ACCESSION   LT726976
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6218)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Mammalian expression vector pPTB-HcRED"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 5218."
                     /db_xref="taxon:1945054"
     regulatory      1..568
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             639..2309
                     /codon_start=1
                     /note="unnamed protein product; hPTB"
                     /protein_id="SJL87097.1"
                     /translation="MDGIVPDIAVGTKRGSDELFSTCVTNGPFIMSSNSASAANGNDSK
                     KFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTE
                     EAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQSGNLA
                     LAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQF
                     QALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYTRPDLP
                     SGDSQPSLDQTMAAAFGAPGIISASPYAGAGFPPTFAIPQAAGLSVPNVHGALAPLAIP
                     SAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVKILFNK
                     KENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQGLTKDYGN
                     SPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLFSSNGGVVKGFKFFQKDR
                     KMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKSTI"
     CDS             2316..3002
                     /codon_start=1
                     /note="unnamed protein product; HcRed1"
                     /protein_id="SJL87098.1"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     polyA_signal    3006..3127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3134..3589)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
     regulatory      3590..3685
                     /regulatory_class="promoter"
                     /note="amp promoter"
     promoter        3616..3720
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    3686..3720
                     /label=5 UTR of amp, incl. RBS
                     /note="5 UTR of amp, incl. RBS"
     promoter        3722..4079
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      3741..4022
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      3930..4065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      3979..4054
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             4114..4908
                     /codon_start=1
                     /note="unnamed protein product; neomycin"
                     /protein_id="SJL87099.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      5099..5168
                     /regulatory_class="terminator"
                     /note="HSV-TK polyA"
     polyA_signal    5140..5187
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5516..6104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5886..6212
                     /label=pMB1 ori
                     /note="pMB1 ori"