LOCUS       Exported               14757 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPSX, complete sequence.
ACCESSION   FJ422118
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Sarovich DS, Pemberton JM.
  TITLE     pPSX: a novel vector for the cloning and heterologous expression of 
            antitumor antibiotic gene clusters
  JOURNAL   Plasmid 57 (3), 306-313 (2007)
  PUBMED    17218012
REFERENCE   2  (bases 1 to 14757)
  AUTHORS   Philip DS, Sarovich DS, Pemberton JM.
  TITLE     Complete sequence and analysis of the stability functions of pPSX, a
            vector that allows stable cloning and expression of Streptomycete 
            genes in Escherichia coli K12
  JOURNAL   Plasmid 62 (1), 39-43 (2009)
  PUBMED    19303899
REFERENCE   3  (bases 1 to 14757)
  AUTHORS   Philip DS, Sarovich DS, Pemberton JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2008) School of Molecular and Microbial Sciences, 
            The University of Queensland, Cooper Road, Brisbane, QLD 4072, 
            Australia
REFERENCE   4  (bases 1 to 14757)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Cloning vector pPSX"
                     /mol_type="other DNA"
                     /db_xref="taxon:630753"
     rep_origin      complement(1..177)
                     /direction=LEFT
                     /label=oriV
                     /note="oriV"
     CDS             complement(282..1253)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA protein"
                     /label=repA
                     /protein_id="ACO89456.1"
                     /translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATM
                     PHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRS
                     QSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNP
                     KRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLR
                     AKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREV
                     LIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
     gene            complement(282..1253)
                     /gene="repA"
                     /label=repA
     CDS             complement(1246..1902)
                     /codon_start=1
                     /gene="resP"
                     /product="ResP protein"
                     /label=resP
                     /protein_id="ACO89457.1"
                     /translation="MPKYYAYLRVSRDGQDPENQKYGLLEYANAKGFAPLQIEEEIASR
                     AKDWRKRKLGAIIEKAERGDVLLTPEITRIAGSALAALEILKAASERGLIVHVTKQKII
                     MDGSLQSDIMATVLGLAAQIERHFIQARTTEALQVARERGKTLGRPKGSKSSALKLDSR
                     IDEVQAYVNLGLPQSRAAELLGVSPHTLRLFIKRRNIKPTNTRPTITMPGREQHA"
     gene            complement(1246..1902)
                     /gene="resP"
                     /label=resP
     CDS             complement(2054..2284)
                     /codon_start=1
                     /gene="orf38"
                     /product="hypothetical protein"
                     /label=orf38
                     /protein_id="ACO89458.1"
                     /translation="MFLILWWLRGPVRFLLGLASFASLITLPIMWLGLDSANKTHIMLS
                     VAGVGLGASALMWFYDALLMRLAPEPILFST"
     gene            complement(2054..2284)
                     /gene="orf38"
                     /label=orf38
     gene            complement(2491..3508)
                     /gene="intI1"
                     /label=intI1
     CDS             complement(2495..3508)
                     /codon_start=1
                     /gene="intI1"
                     /product="IntI1 integrase"
                     /label=intI1
                     /protein_id="ACO89459.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVNWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
     promoter        3393..3421
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     gene            3743..3983
                     /gene="dfrB2"
                     /label=dfrB2
     CDS             3747..3983
                     /codon_start=1
                     /gene="dfrB2"
                     /product="dihydrofolate reductase"
                     /label=dfrB2
                     /protein_id="ACO89460.1"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     misc_feature    complement(4134..4580)
                     /note="lac-operon; includes MCS"
     protein_bind    4237..4258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4273..4303
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4311..4327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4335..4351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4347..4631
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLITSEFD"
     misc_feature    4360..4415
                     /label=MCS
                     /note="MCS"
     misc_feature    4361..4417
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4421..4437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4761..7139
                     /label=lambda cos
                     /note="lambda cos"
     terminator      4837..4931
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             7749..8096
                     /codon_start=1
                     /gene="qacEdelta1"
                     /product="QacEdelta1"
                     /label=qacEdelta1
                     /protein_id="ACO89461.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
     gene            7749..8096
                     /gene="qacEdelta1"
                     /label=qacEdelta1
     CDS             8090..8929
                     /codon_start=1
                     /gene="sul1"
                     /product="dihydropteroate synthase"
                     /label=sul1
                     /protein_id="ACO89462.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG
                     PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ
                     GFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSALRRS
                     GVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLP
                     VKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGLDHA"
     gene            8090..8929
                     /gene="sul1"
                     /label=sul1
     CDS             9375..9719
                     /codon_start=1
                     /gene="orf23"
                     /product="hypothetical protein"
                     /label=orf23
                     /protein_id="ACO89463.1"
                     /translation="MLVLTTEGRKRRQPPGQINAGVKAMKRKFRLGQMLATIGVLELCK
                     HKHIDLGPLIDRHVSGDWGDVDDAQREANEEAVKECGTIVSVYHPHGVRVLIVTDGDRS
                     HTVAMLPHEY"
     gene            9375..9719
                     /gene="orf23"
                     /label=orf23
     CDS             9735..10388
                     /codon_start=1
                     /gene="orf22"
                     /product="hypothetical protein"
                     /label=orf22
                     /protein_id="ACO89464.1"
                     /translation="MSRKHQPKTERQEKAAVIAASLPEDRGELMDAAAEAIRQYDAAIV
                     GCDDDAAHAARDRYEAVIWKLNGNSFFGTKADADSPGYQVERHCAATPGTVPLWGQKGE
                     FLMTVEGIRAVVEFGNGYGSMYAHFAFHAVDLDLPFISETGYRSHFTPVMGGMTVDEAA
                     EAIMRAILAEKGRVLIKPDSRQFYEGREARAWLDYTRPAQTIYQEGNGQIAFGF"
     gene            9735..10388
                     /gene="orf22"
                     /label=orf22
     CDS             10536..10787
                     /codon_start=1
                     /gene="orf21"
                     /product="hypothetical protein"
                     /label=orf21
                     /protein_id="ACO89465.1"
                     /translation="MTNEHTAPVLFYFDKAETLREFEAFRVEASQITRPHQIPAQVEVW
                     NVIGKRRFIDRQEVIAEFPNELYAQIFADMADKTAAHI"
     gene            10536..10787
                     /gene="orf21"
                     /label=orf21
     CDS             complement(10941..11327)
                     /codon_start=1
                     /gene="orf20"
                     /product="hypothetical protein"
                     /label=orf20
                     /protein_id="ACO89466.1"
                     /translation="MAGPQTTREALIAELLGDVGVLIDKTDELRASFPAAADEAAAKVK
                     AAGQRATEDVATAATQLTRDLATQTEKLLQGVQQAAREAQAAARVVDRSARRFALLATA
                     MGLAAGIVGGVLAGLALSGYSFGA"
     gene            complement(10941..11327)
                     /gene="orf20"
                     /label=orf20
     gene            complement(11340..12036)
                     /gene="orf19"
                     /label=orf19
     CDS             complement(11341..12036)
                     /codon_start=1
                     /gene="orf19"
                     /product="hypothetical protein"
                     /label=orf19
                     /protein_id="ACO89467.1"
                     /translation="MKVAVINFSGNVGKSTVAQHLLAPRMPNAEFVAVESINADEGTGE
                     VVRGKQFGQLQEQLLMVDEAVIDIGASNVEDFVKLMQQYRGSHEDFDYFVVPAVKESKQ
                     IKDTIATINALSKMGVPAKKIRVVFNKLESDETVEEAFYPLFAYHDDSKAFTIKPKAAI
                     QFSELYHKLRTYRVTIEQLLTDETDYKAKLREAKNPEEKAQAAAMISMRRLAFSAKENL
                     DAVYAAISK"
     CDS             complement(12033..12458)
                     /codon_start=1
                     /gene="orf18"
                     /product="hypothetical protein"
                     /label=orf18
                     /protein_id="ACO89468.1"
                     /translation="MNETDALKDRIENIRPRMTLAAKLRELMPEIDRQVRAGVQHDDIV
                     ETLNANGFDVNLNTFRSYLYRYRKKARASEGQPEPAQATPKADGNSPVVSGGPGDVSPD
                     FDADSLADALDARKRDELGDKYLGLKRHIFSKRSDNK"
     gene            complement(12033..12458)
                     /gene="orf18"
                     /label=orf18
     oriT            complement(12580..12981)
                     /direction=LEFT
     gene            complement(12912..13097)
                     /gene="trwA"
                     /label=trwA
                     /note="TrwA; fragment"
     CDS             complement(13177..13701)
                     /codon_start=1
                     /gene="nuc"
                     /product="Nuc protein"
                     /label=nuc
                     /protein_id="ACO89469.1"
                     /translation="MKSALAALRAVAAAVVLIVSVPAWADFRGEVVRILDGDTIDVLVN
                     RQTIRVRLADIDAPESGQAFGSRARQRLADLTFRQEVQVTEKEVDRYGRTLGVVYAPLQ
                     YPGGQTQLTNINAIMVQEGMAWAYRYYGKPTDAQMYEYEKEARRQRLGLWSDPNAQEPW
                     KWRRASKNATN"
     gene            complement(13177..13701)
                     /gene="nuc"
                     /label=nuc
     CDS             complement(13698..14327)
                     /codon_start=1
                     /gene="orf35"
                     /product="hypothetical protein"
                     /label=orf35
                     /protein_id="ACO89470.1"
                     /translation="MAITKQDIWRAADELDAEGIRPTLAAVRKKLGSGSFTTISDAMAE
                     WKNRKTATLPSSDPLPVAVNEHLAELGNALWAIALAHANARFDEDRKQIEADKAAISQQ
                     LAEAIELADTFTRENDQLRERVNQLEPMERERDKLADQLAEVKRRSGEELNRCMEKLTQ
                     RDNEAIEARKQAKEAIERAASLQGQVEALKEQVANLTAVLKTGGKQ"
     gene            complement(13698..14327)
                     /gene="orf35"
                     /label=orf35
     misc_feature    14022..14023
                     /note="IS1071 was inserted at this position during 
                     transposition experiments, the CTGAT sequence (14023-14027)
                     is a by-product of the transposition (Sota,M., Yano,H., 
                     Nagata,Y., Ohtsubo,Y., Genka,H., Anbutsu,H.,Kawasaki,H. and
                     Tsuda,M.Functional analysis of unique class II insertion 
                     sequence IS1071 Appl. Environ. Microbiol. 72 (1), 291-297 
                     (2006))"
     rep_origin      14567..245
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"