LOCUS       Exported                6348 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Plastid transformation vector pPRV312L, complete sequence.
ACCESSION   DQ489715
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Chakrabarti SK, Lutz KA, Lertwiriyawong B, Svab Z, Maliga P.
  TITLE     Expression of the cry9Aa2 B.t. gene in tobacco chloroplasts confers 
            resistance to potato tuber moth
  JOURNAL   Transgenic Res. 15 (4), 481-488 (2006)
  PUBMED    16906448
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Maliga P, Lutz KA.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2006) Waksman Institute, Rutgers University, 190 
            Frelinghuysen Road, Piscataway, NJ 08854-8020, USA
REFERENCE   3  (bases 1 to 6348)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Plastid transformation vector pPRV312L"
                     /mol_type="other DNA"
                     /db_xref="taxon:384036"
     misc_feature    1..1242
                     /note="similar to Nicotiana tabacum ptDNA in GenBank 
                     Accession Number Z00044; plastid sequence targeting 
                     insertions in the trnI-trnA intergenic region"
     misc_feature    1243..1331
                     /note="multiple cloning site; includes SwaI, EcoRI, SacI, 
                     KpnI, SmaI, BamHI, XbaI, SalI, PstI, SphI, HindIII and NotI
                     restriction sites"
     misc_feature    1259..1315
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1332..1365
                     /label=P1 phage loxP
                     /note="P1 phage loxP"
     protein_bind    1332..1365
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1372..1544
                     /note="tobacco plastid rRNA operon promoter (Prrn) fused 
                     with tobacco plastid atpB gene leader (described in Kuroda 
                     and Maliga, Plant Phys 125:430-436, 2001)"
     regulatory      1372..1455
                     /regulatory_class="promoter"
                     /note="tobacco plastid rRNA operon promoter (Prrn)"
     misc_feature    1455..1544
                     /label=tobacco plastid atpB gene leader
                     /note="tobacco plastid atpB gene leader"
     CDS             1545..2411
                     /codon_start=1
                     /gene="atpB-aad-c-myc"
                     /product="AtpB-AAD-c-myc fusion protein"
                     /label=atpB-aad-c-myc
                     /note="aminoglycoside 3-adenylyltransferase; confers 
                     resistance to spectinomycin and streptomycin by 
                     adenylation; contains C-terminal c-myc tag"
                     /protein_id="ABI34135.1"
                     /translation="MRINPTTSGSGVSTASSEAVIAEVSTQLSEVVGVIERHLEPTLLA
                     VHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVT
                     IVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGP
                     AAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDV
                     AADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGKEQK
                     LISEEDL"
     gene            1545..2411
                     /gene="atpB-aad-c-myc"
                     /label=atpB-aad-c-myc
     misc_feature    1545..1586
                     /gene="atpB-aad-c-myc"
                     /label=N-terminus of tobacco plastid AtpB
                     /note="N-terminus of tobacco plastid AtpB"
     misc_feature    1596..2378
                     /gene="atpB-aad-c-myc"
                     /label=AadA coding sequence
                     /note="AadA coding sequence"
     CDS             1596..2378
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="EAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSD
                     IDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRE
                     LQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEA
                     LNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVI
                     LEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2379..2408
                     /gene="atpB-aad-c-myc"
                     /label=c-myc tag
                     /note="c-myc tag"
     CDS             2379..2408
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    2425..2613
                     /note="similar to tobacco plastid psbA 3 UTR in GenBank 
                     Accession Number Z00044"
     misc_feature    2632..2665
                     /label=P1 phage loxP
                     /note="P1 phage loxP"
     protein_bind    2632..2665
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2674..2681
                     /label=contains SwaI restriction site
                     /note="contains SwaI restriction site"
     misc_feature    2685..3508
                     /note="similar to Nicotiana tabacum ptDNA in GenBank 
                     Accession Number Z00044; plastid sequence targeting 
                     insertions in the trnI-trnA intergenic region"
     misc_feature    3509..6348
                     /note="pUC118 vector sequence; similar to GenBank Accession
                     Number U07649"
     rep_origin      3651..4106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4388..4492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4493..5353
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5524..6112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"