LOCUS       Exported                4733 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPROEX-1, complete sequence.
ACCESSION   U19585
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Polayes D.
  TITLE     Plasmid pPROEX - cloning vector for protein expression and 
            purification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Polayes D, Hughes AJohn.
  TITLE     Efficient protein expression and simple purification using the 
            pPROEX-1 system
  JOURNAL   Focus 16, 81-84 (1994)
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Horton ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-1995) Mary E. Horton, Technical Services, Life 
            Technologies, Inc., 8400 Helgerman Court, Gaithersburg, MD 
            20884-9980, USA
REFERENCE   4  (bases 1 to 4733)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Cloning vector pPROEX-1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:39034"
     regulatory      193..266
                     /regulatory_class="promoter"
                     /gene="Trc"
                     /note="expression in Escherichia coli under regulation of 
                     the lac repressor"
                     /citation="[2]"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     gene            193..266
                     /gene="Trc"
                     /label=Trc
     protein_bind    230..246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             312..332
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      625..711
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      803..830
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        850..941
                     /gene="bla"
                     /label=AmpR promoter
     CDS             942..1802
                     /codon_start=1
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=beta-lactamase
                     /citation="[2]"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAA67273.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1973..2561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2564..2571
                     /standard_name="ORI Escherichia coli pMB1"
                     /label=ORI Escherichia coli pMB1
                     /citation="[2]"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     rep_origin      complement(2809..3237)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3534..3611
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3612..4694
                     /codon_start=1
                     /product="Lac repressor"
                     /label=Lac repressor
                     /citation="[2]"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAA67274.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"