LOCUS       Exported                9502 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pPP1mds1, complete sequence.
ACCESSION   LT727222
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9502)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9502
                     /organism="Yeast expression vector pPP1mds1"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4484."
                     /db_xref="taxon:1945494"
     promoter        2..937
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     regulatory      7..772
                     /regulatory_class="promoter"
                     /note="AOX1 promoter"
     misc_feature    773..937
                     /label=5 UTR of AOX1
                     /note="5 UTR of AOX1"
     CDS             954..2525
                     /codon_start=1
                     /note="unnamed protein product; mds1"
                     /protein_id="SJL87800.1"
                     /translation="MRFPSSSVLALGLIGPALAYPKPGATKRGSPNPTRAAAVKAAFQT
                     SWNAYHHFAFPHDDLHPVSNSFDDERNGWGSSAIDGLDTAILMGDADIVNTILQYVPQI
                     NFTTTAVANQGSSVFETNIRYLGGLLSAYDLLRGPFSSLATNQTLVNSLLRQAQTLANG
                     LKVAFTTPSGVPDPTVFFNPTVRRSGASSNNVAEIGSLVLEWTRLSDLTGNPQYAQLAQ
                     KGESYLLNPKGSPEAWPGLIGTFVSTSNGTFQDSSGSWSGLMDSFYEYLIKMYLYDPVA
                     FAHYKDRWVLGADSTIGHLGSHPSTRKDLTFLSSYNGQSTSPNSGHLASFGGGNFILGG
                     ILLNEQKYIDFGIKLASSYFGTYTQTASGIGPEGFAWVDSVTGAGGSPPSSQSGFYSSA
                     GFWVTAPYYILRPETLESLYYAYRVTGDSKWQDLAWEALSAIEDACRAGSAYSSINDVT
                     QANGGGASDDMESFWFAEALKYAYLIFAEESDVQVQATGGNKFVFNTEAHPFSIRSSSR
                     RGGHLA"
     regulatory      2800..3059
                     /regulatory_class="terminator"
                     /note="AOX1 terminator"
     terminator      2800..3046
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(3459..5993)
                     /codon_start=1
                     /note="unnamed protein product; HIS4"
                     /protein_id="SJL87801.1"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     misc_feature    6348..7105
                     /label=3 flanking region of AOX1
                     /note="3 flanking region of AOX1"
     misc_feature    6348..7104
                     /label=AOX1 3 fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    7247..7387
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7389..7791)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(7573..8161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8332..9192)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87802.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9193..9297)
                     /gene="bla"
                     /label=AmpR promoter