LOCUS       Exported                4975 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPolh-1629, complete sequence.
ACCESSION   KU749550
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Liu X.
  TITLE     A Highly Efficient and Simple Construction Strategy for Producing 
            Recombinant Baculovirus Bombyx mori Nucleopolyhedrovirus
  JOURNAL   PLoS ONE 11 (3), E0152140 (2016)
  PUBMED    27008267
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2016) Biotechnology Research Institute, Chinese 
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, 
            Beijing, Beijing 100081, China
REFERENCE   3  (bases 1 to 4975)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Cloning vector pPolh-1629"
                     /mol_type="other DNA"
                     /db_xref="taxon:1822055"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    434..864
                     /label=homologous arm for BmNPV polyhedrin gene
                     /note="homologous arm for BmNPV polyhedrin gene"
     promoter        878..906
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             954..2144
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance protein"
                     /label=tetR
                     /protein_id="AMS25563.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            954..2144
                     /gene="tetR"
                     /label=tetR
     misc_feature    2232..2693
                     /label=homologous arm for BmNPV orf1629 gene
                     /note="homologous arm for BmNPV orf1629 gene"
     primer_bind     complement(2754..2770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2778..2794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2802..2832)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2847..2868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3156..3744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3915..4775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4776..4880)
                     /gene="bla"
                     /label=AmpR promoter