LOCUS       Exported                8372 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range shuttle vector pPMQAK1, complete sequence.
ACCESSION   GU933126
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Huang HH, Camsund D, Lindblad P, Heidorn T.
  TITLE     Design and characterization of molecular tools for a Synthetic 
            Biology approach towards developing cyanobacterial biotechnology
  JOURNAL   Nucleic Acids Res. 38 (8), 2577-2593 (2010)
  PUBMED    20236988
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Huang H-H., Camsund D, Lindblad P, Heidorn T.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2010) Dept. of Photochemistry & Molecular Science,
            Uppsala University, P.O. Box 523, Uppsala, Uppland S-75120, Sweden
REFERENCE   3  (bases 1 to 8372)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Broad host range shuttle vector pPMQAK1"
                     /mol_type="other DNA"
                     /db_xref="taxon:748448"
     CDS             complement(823..1683)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance protein"
                     /protein_id="ADE33911.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(823..1683)
                     /gene="bla"
                     /label=bla
     promoter        complement(1684..1788)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1805..1825
                     /note="binding site for standard Biobrick forward primer 
                     VF2 (BBa_G00100)"
     terminator      1878..1921
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      1895..1914
                     /regulatory_class="terminator"
     misc_feature    1924..1945
                     /note="upstream region of standard cloning site for use 
                     with BioBricks; includes EcoRI & XbaI sites"
     misc_feature    1946..2620
                     /note="derived from BioBrick CcdB toxin region (BBa_P1010)"
     CDS             complement(1974..2279)
                     /codon_start=1
                     /gene="ccdB"
                     /product="DNA gyrase toxin CcdB"
                     /label=ccdB
                     /protein_id="ADE33912.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1974..2279)
                     /gene="ccdB"
                     /label=ccdB
     misc_feature    2621..2641
                     /note="downstream region of standard cloning site for use 
                     with BioBricks; includes SpeI & PstI sites"
     terminator      2642..2699
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     regulatory      2650..2682
                     /regulatory_class="terminator"
     primer_bind     2775..2794
                     /note="binding site for standard Biobrick reverse primer VR
                     (BBa_G00101)"
     rep_origin      2881..3275
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     rep_origin      2885..3275
                     /note="oriV; origin of vegetative replication"
     CDS             complement(3302..3586)
                     /codon_start=1
                     /gene="mobC"
                     /product="mobilization protein C"
                     /label=mobC
                     /protein_id="ADE33913.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            complement(3302..3586)
                     /gene="mobC"
                     /label=mobC
     oriT            3617..3704
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             3785..5914
                     /codon_start=1
                     /gene="mobA"
                     /product="mobilization protein A"
                     /label=mobA
                     /protein_id="ADE33914.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
     gene            3785..5914
                     /gene="mobA"
                     /label=mobA
     CDS             4533..4946
                     /codon_start=1
                     /gene="mobB"
                     /product="mobilization protein B"
                     /label=mobB
                     /protein_id="ADE33915.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            4533..4946
                     /gene="mobB"
                     /label=mobB
     CDS             4943..5914
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B"
                     /label=repB
                     /protein_id="ADE33916.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     gene            4943..5914
                     /gene="repB"
                     /label=repB
     CDS             5975..6187
                     /codon_start=1
                     /product="unknown protein E"
                     /label=unknown protein E
                     /protein_id="ADE33917.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             6189..6395
                     /codon_start=1
                     /product="repressor protein F"
                     /label=repressor protein F
                     /protein_id="ADE33918.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             6425..7264
                     /codon_start=1
                     /gene="repA"
                     /product="regulatory protein A"
                     /label=repA
                     /protein_id="ADE33919.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     gene            6425..7264
                     /gene="repA"
                     /label=repA
     CDS             7251..8102
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C"
                     /label=repC
                     /protein_id="ADE33920.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     gene            7251..8102
                     /gene="repC"
                     /label=repC
     CDS             complement(8225..668)
                     /codon_start=1
                     /gene="aph"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph
                     /note="kanamycin resistance protein"
                     /protein_id="ADE33910.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(8225..668)
                     /gene="aph"
                     /label=aph