LOCUS       Exported                5917 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Synthetic construct cloning vector pPLuc, complete sequence.
ACCESSION   AB037684
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Chen A, Kao YF, Brown CM.
  TITLE     Translation of the first upstream ORF in the hepatitis B virus 
            pregenomic RNA modulates translation at the core and polymerase 
            initiation codons
  JOURNAL   Nucleic Acids Res. 33 (4), 1169-1181 (2005)
  PUBMED    15731337
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Chen AB, Kao AY-F., Brown CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2000) Chris M. Brown, University of Otago, 
            Department of Biochemistry; P.O.Box 56, Dunedin, Otago 9001, New 
            Zealand (E-mail:chris.brown@stonebow.otago.ac.nz, 
            Tel:+64-3-479-7875, Fax:+64-3-479-7866)
REFERENCE   3  (bases 1 to 5917)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /label=cloning vector pPLuc
                     /note="cloning vector pPLuc"
                     /db_xref="taxon:32630"
     misc_feature    1..495
                     /label=HBV Adw 5 leader of pregenomic RNA
                     /note="HBV Adw 5 leader of pregenomic RNA"
     CDS             35..94
                     /codon_start=1
                     /gene="C0"
                     /label=C0
                     /note="unnamed protein product; ORF"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="BAA93573.1"
                     /translation="MSHCSSLQAVPWVALGHGH"
     gene            35..94
                     /gene="C0"
                     /label=C0
     CDS             84..581
                     /codon_start=1
                     /product="modified C"
                     /label=modified C
                     /protein_id="BAA93574.1"
                     /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESP
                     EHCSPHHTALRQAILCWGELMTLATWVGNNLEDPATRDLVVNYVNTNMGLKIRQLLWFH
                     ISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNARRRRRQKHKERPGAILSAGRWNRWRA
                     TA"
     CDS             490..2151
                     /codon_start=1
                     /gene="PLuc"
                     /product="luciferase"
                     /label=PLuc
                     /note="P codon luciferase fusion"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="BAA93575.1"
                     /translation="MPDVEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAF
                     TDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVA
                     VAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRF
                     SHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQ
                     DYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIR
                     QGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPM
                     IMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELE
                     SILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRG
                     GVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     gene            490..2151
                     /gene="PLuc"
                     /label=PLuc
     CDS             502..2151
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2361..2482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3147..3735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3906..4766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4767..4871)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4898..5353
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    5484..5532
                     /note="synthetic polyadenylation signal"
     misc_feature    5546..5637
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        5696..5892
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5743..5878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      5899..5917
                     /regulatory_class="promoter"
                     /function="T7 promoter"
                     /note="derived from pGLControl (Promega)"
     promoter        5899..5917
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"