LOCUS       Exported               14033 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLEX-5031, complete sequence.
ACCESSION   AY225223
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14033)
  AUTHORS   Schunmann PHD., Surin B, Waterhouse PM.
  TITLE     A suite of novel promoters and terminators for plant biotechnology. 
            II. The pPLEX series for use in monocots
  JOURNAL   Funct. Plant Biol. 30, 453-460 (2003)
REFERENCE   2  (bases 1 to 14033)
  AUTHORS   Schunmann PHD.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2003) CSIRO Plant Industry, GPO Box 1600, 
            Canberra, ACT 2617, Australia
REFERENCE   3  (bases 1 to 14033)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14033
                     /organism="Cloning vector pPLEX-5031"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:220616"
     regulatory      21..565
                     /regulatory_class="promoter"
                     /note="S4 promoter derived from subterranean clover stunt 
                     virus DNA segment 4"
     intron          609..1618
                     /number=1
                     /note="ubiquitin; Ubi1"
     misc_feature    1639..1680
                     /note="multiple cloning site; MCS"
     regulatory      1681..2574
                     /regulatory_class="terminator"
                     /note="Me1 terminator derived from Flavaria bidentis NADP 
                     malic gene"
     regulatory      2635..3076
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter from pIG121Hm"
     promoter        2723..3067
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3077..3409,3600..4292)
                     /codon_start=1
                     /product="hygromycin"
                     /label=hygromycin
                     /note="HPT"
                     /protein_id="AAP23188.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          3410..3599
                     /note="modified CAT-1"
     intron          3416..3605
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     regulatory      4317..4593
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      4333..4585
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(4655..4838)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4691..4870)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4877..5498
                     /label=RB (right border)
                     /note="RB (right border)"
     misc_feature    4994..5018
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5520..6134
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      5611..6142
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(6817..7605)
                     /codon_start=1
                     /product="spectinomycin resistant protein"
                     /label=spectinomycin resistant protein
                     /protein_id="AAP23189.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8702..9290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10650..11798)
                     /codon_start=1
                     /product="TrfA"
                     /label=TrfA
                     /protein_id="AAP23190.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  12423..13190
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     oriT            13250..13359
                     /note="incP origin of transfer"
     misc_feature    13499..13990
                     /label=LB (left border)
                     /note="LB (left border)"
     misc_feature    13914..13938
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"