LOCUS       Exported               13535 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLEX-5023, complete sequence.
ACCESSION   AY225222
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Schunmann PHD., Surin B, Waterhouse PM.
  TITLE     A suite of novel promoters and terminators for plant biotechnology. 
            II. The pPLEX series for use in monocots
  JOURNAL   Funct. Plant Biol. 30, 453-460 (2003)
REFERENCE   2  (bases 1 to 13535)
  AUTHORS   Schunmann PHD.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2003) CSIRO Plant Industry, GPO Box 1600, 
            Canberra, ACT 2617, Australia
REFERENCE   3  (bases 1 to 13535)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Cloning vector pPLEX-5023"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:217652"
     regulatory      23..576
                     /regulatory_class="promoter"
                     /note="S7 promoter derived from subterranean clover stunt 
                     virus DNA segment 7"
     intron          594..1128
                     /number=1
                     /note="alcohol dehydrogenase; Adh1"
     misc_feature    1141..1182
                     /note="multiple cloning site; MCS"
     regulatory      1183..2076
                     /regulatory_class="terminator"
                     /note="Me1 terminator derived from Flavaria bidentis NADP 
                     malic gene"
     regulatory      2137..2578
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter from pIG121Hm"
     promoter        2225..2569
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(2579..2911,3102..3794)
                     /codon_start=1
                     /product="hygromycin"
                     /label=hygromycin
                     /note="HPT"
                     /protein_id="AAP23185.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     intron          2912..3101
                     /note="modified CAT-1"
     intron          2918..3107
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     regulatory      3819..4095
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      3835..4087
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(4157..4340)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4193..4372)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4379..5000
                     /label=RB (right border)
                     /note="RB (right border)"
     misc_feature    4496..4520
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5022..5636
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      5113..5644
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(6319..7107)
                     /codon_start=1
                     /product="spectinomycin resistant protein"
                     /label=spectinomycin resistant protein
                     /protein_id="AAP23186.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8204..8792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10152..11300)
                     /codon_start=1
                     /product="TrfA"
                     /label=TrfA
                     /protein_id="AAP23187.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  11925..12692
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     oriT            12752..12861
                     /note="incP origin of transfer"
     misc_feature    13001..13492
                     /label=LB (left border)
                     /note="LB (left border)"
     misc_feature    13416..13440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"