LOCUS       Exported               12880 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLEX-4004, complete sequence.
ACCESSION   AY159034
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Schunmann PHD., Llewellyn DJ, Surin B, Boevink P, De Feyter RC, 
            Waterhouse PM.
  TITLE     A suite of novel promoters and terminators for plant biotechnology
  JOURNAL   Funct. Plant Biol. 30, 443-452 (2003)
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   Schunmann PHD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2002) Plant Industry, CSIRO, Clunies Ross Street, 
            Canberra, ACT 2602, Australia
REFERENCE   3  (bases 1 to 12880)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Cloning vector pPLEX-4004"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:217651"
     regulatory      23..1068
                     /regulatory_class="promoter"
                     /note="S7S7 promoter derived from subterranean clover stunt
                     virus DNA segment 7"
     misc_feature    1069..1116
                     /note="multiple cloning site; MCS"
     regulatory      1117..2010
                     /regulatory_class="terminator"
                     /note="Me1 terminator derived from Flavaria bidentis NADP 
                     malic enzyme gene"
     misc_feature    2069..2690
                     /label=RB (right border)
                     /note="RB (right border)"
     misc_feature    2186..2210
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      2711..3325
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      2802..3333
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(4008..4796)
                     /codon_start=1
                     /product="spectinomycin resistant protein"
                     /label=spectinomycin resistant protein
                     /protein_id="AAP22327.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5893..6481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7841..8989)
                     /codon_start=1
                     /product="TrfA"
                     /label=TrfA
                     /protein_id="AAP22328.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  9614..10381
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     oriT            10441..10550
                     /note="incP origin of transfer"
     misc_feature    10690..11180
                     /label=LB (left border)
                     /note="LB (left border)"
     misc_feature    11105..11129
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      11213..11735
                     /regulatory_class="promoter"
                     /note="S1 promoter derived from subterranean clover stunt 
                     virus DNA segment 1"
     CDS             11745..12539
                     /codon_start=1
                     /product="neomycin phosphotransferase II"
                     /label=neomycin phosphotransferase II
                     /note="kanamycin resistance"
                     /protein_id="AAP21560.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      12721..12859
                     /regulatory_class="terminator"
                     /note="S3 terminator derived from subterranean clover stunt
                     virus DNA segment 3"