LOCUS       Exported                3198 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pPLcA1, complete sequence.
ACCESSION   LT727519
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Bacterial expression vector pPLcA1"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2469."
                     /db_xref="taxon:1944704"
     misc_feature    98..130
                     /label=5 UTR of MS2 polymerase, incl. RBS
                     /note="5 UTR of MS2 polymerase, incl. RBS"
     CDS             131..682
                     /codon_start=1
                     /note="unnamed protein product; HBc"
                     /protein_id="SJL88720.1"
                     /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESP
                     EHCSPHHTALRQAILCWGELMTLATWVGVNLEDPASRDLVVSYVNTNMGLKFRQLLWFH
                     ISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRGRSPRRRTPSP
                     RRRRSQSPRRRRSQSRESQC"
     regulatory      686..725
                     /regulatory_class="terminator"
                     /note="synthetic polyA"
     rep_origin      complement(781..1183)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(965..1553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1724..2584)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL88721.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      2950..3080
                     /regulatory_class="promoter"
                     /note="lambda PL promoter"