LOCUS       Exported                8742 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPlat-Pds-Mod4.1, complete sequence.
ACCESSION   DQ404589
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Steinbrenner J, Sandmann G.
  TITLE     Transformation of the green alga Haematococcus pluvialis with a 
            phytoene desaturase for accelerated astaxanthin biosynthesis
  JOURNAL   Appl. Environ. Microbiol. 72 (12), 7477-7484 (2006)
  PUBMED    17012596
REFERENCE   2  (bases 1 to 8742)
  AUTHORS   Steinbrenner J, Sandmann G.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2006) Plant Physiology and Biochemistry, 
            University of Konstanz, Universitaetsstr. 10, Konstanz 78457, 
            Germany
REFERENCE   3  (bases 1 to 8742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     Name: pPlat-Pds-Mod4.1.
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Cloning vector pPlat-Pds-Mod4.1"
                     /lab_host="Haematococcus pluvialis"
                     /mol_type="other DNA"
                     /label=cloning and transformation vector
                     /note="cloning and transformation vector"
                     /db_xref="taxon:391007"
     gene            134..5825
                     /gene="pdsMod4.1"
                     /label=pdsMod4.1
     regulatory      134..2140
                     /regulatory_class="promoter"
                     /gene="pdsMod4.1"
                     /note="derived from the phytoene desaturase gene"
     mRNA            join(2141..2510,2600..2712,2818..3184,3537..3671,3965..4043,
                     4394..4743,4974..5044,5222..5825)
                     /gene="pdsMod4.1"
                     /product="phytoene desaturase"
                     /label=pdsMod4.1 mRNA
     CDS             join(2181..2510,2600..2712,2818..3184,3537..3671,3965..4043,
                     4394..4743,4974..5044,5222..5489)
                     /codon_start=1
                     /gene="pdsMod4.1"
                     /product="phytoene desaturase"
                     /label=pdsMod4.1
                     /protein_id="ABG29431.1"
                     /translation="MQTTMRGQASGSGCTSGRQARGHWSRRSVRERGALRVVAKDYPTP
                     DFQSSDTYQEALSLSTKLRNAPRPAKPLRVVIAGAGLAGLSAAKYLADAGHHPVVLEGR
                     DVLGGKVAAWKDEDGDWYETGLHIFFGAYPNIQNLFKELGIQDRLQWKEHSMIFAMPDA
                     PGEFSRFDFPELPAPWNGIIAILRNNQMLSWPEKIRFRIGLLPAIIFGQRYCEEQDELT
                     VTEWMRKQGVPDRVNEEVFIAMAKALNFINPDDLSMTVVLTALNRFLQEQHGSKMAFLD
                     GAPPERLCQPMVDYFKARGGDLMFNSRVKQIVLNDDKSVKHLALTNGQTVEGDLYISAM
                     PVDIMKILMPDPWASMPYFKQLNGLEGVPVINIHIWFDRKLTTVDHLLFSRSPLLSVYA
                     DMSTTSKEYRDDKKSMLELVFAPAKEWIGRPDEEIIAATMTELERLFPTEVRADQSMAK
                     ILKYKVVKTPLSVYKSTAGREKFRPTQRSPISNFYLAGDYTKQKYRASMEGAVFSGKLV
                     TEAIVEDWSARGVTSSAASRQPALAAAGVVGRVGSSDWARWLQPVGAIAGGCK"
     CDS             complement(6238..6600)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWKL"
     primer_bind     6387..6403
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6410..6428
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6437..6544
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     misc_feature    6441..6543
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     6454..6470
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6504..6520)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6557..6575)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6596..6612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6620..6636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6644..6674)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6689..6710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      6816..7996
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(6998..7586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7757..8617)
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /protein_id="ABG29430.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(7757..8617)
                     /gene="amp"
                     /label=amp
     promoter        complement(8618..8722)
                     /gene="bla"
                     /label=AmpR promoter