LOCUS       Exported                6783 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pPLaACR26, complete sequence.
ACCESSION   LT727439
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6783)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Bacterial expression vector pPLaACR26"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 311."
                     /db_xref="taxon:1944690"
     regulatory      complement(117..247)
                     /regulatory_class="promoter"
                     /note="PL promoter"
     CDS             615..1430
                     /codon_start=1
                     /note="unnamed protein product; kanamycin resistance"
                     /protein_id="SJL88456.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(1716..2107)
                     /direction=LEFT
                     /label=ColE1 ori
                     /note="ColE1 ori"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2965..3154)
                     /label=insertion element 1
                     /note="insertion element 1"
     CDS             complement(3491..5128)
                     /codon_start=1
                     /note="unnamed protein product; POL"
                     /protein_id="SJL88457.1"
                     /translation="MSKTTKKFNSLCIDLPRDLSLEIYQSIASVATGSGDPHSDDFTAI
                     AYLRDELLTKHPTLGSGNDEATRRTLAIAKLREANGDRGQINREGFLHDKSLSWDPDVL
                     QTSIRSLIGNLLSGYRSSLFGQCTFSNGAPMGHKLQDAAPYKKFAEQATVTPRALRAAL
                     LVRDQCAPWIRHAVRYNESYEFRLVVGNGVFTVPKNNKIDRAACKEPDMNMYLQKGVGA
                     FIRRRLKSVGIDLNDQSINQRLAQQGSVDGSLATIDLSSASDSISDRLVWSFLPPELYS
                     YLDRIRSHYGIVDGETIRWELFSTMGNGFTFELESMIFWAIVKATQIHFGNAGTIGIYG
                     DDIICPSEIAPRVLEALAYYGFKPNLRKTFVSGLFRESCGAHFYRGVDVKPFYIKKPVD
                     NLFALMLILNRLRGWGVVGGMSDPRLYKVWVRLSSQVPSMFFGGTDLAADYYVVSPPTA
                     VSVYTKTPYGRLLADTRTSGFRLARIARERKFFSEKHDSGRYIAWFHTGGEITDSMKSA
                     GVRVIRTSEWLTPVPTFPQECGPASSPR"
     CDS             complement(4984..5211)
                     /codon_start=1
                     /note="unnamed protein product; LYS"
                     /protein_id="SJL88458.1"
                     /translation="METRFPQQSQQTPASTNRRRPFKHEDYPCRRQQRSSTLYVLIFLA
                     IFLSKFTNQLLLSLLEAVIRTVTTLQQLLT"
     misc_feature    complement(5129..5161)
                     /label=5 UTR of POL
                     /note="5 UTR of POL"
     CDS             complement(5162..5554)
                     /codon_start=1
                     /note="unnamed protein product; COAT"
                     /protein_id="SJL88459.1"
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             complement(5162..5551)
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     misc_feature    complement(5555..5577)
                     /label=5 UTR of COAT
                     /note="5 UTR of COAT"
     CDS             complement(5578..6759)
                     /codon_start=1
                     /note="unnamed protein product; A"
                     /protein_id="SJL88460.1"
                     /translation="MRAFSTLDRENETFVPSVRVYADGETEDNSFSLKYRSNWTPGRFN
                     STGAKTKQWHYPSPYSRGALSVTSIDQGAYKRSGSSWGRPYEEKAGFGFSLDARSCYSL
                     FPVSQNLTYIEVPQNVANRASTEVLQKVTQGNFNLGVALAEARSTASQLATQTIALVKA
                     YTAARRGNWRQALRYLALNEDRKFRSKHVAGRWLELQFGWLPLMSDIQGAYEMLTKVHL
                     QEFLPMRAVRQVGTNIKLDGRLSYPAANFQTTCNISRRIVIWFYINDARLAWLSSLGIL
                     NPLGIVWEKVPFSFVVDWLLPVGNMLEGLTAPVGCSYMSGTVTDVITGESIISVDAPYG
                     WTVERQGTAKAQISAMHRGVQSVWPTTGAYVKSPFSMVHTLDALALIRQRLSR"
     misc_feature    complement(6760..6783)
                     /label=5 UTR of A
                     /note="5 UTR of A"