LOCUS       Exported               13717 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPIPRA589, complete sequence.
ACCESSION   JQ284302
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13717)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
  TITLE     Enabling Technologies for Plant Transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13717)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Plant Sciences, UC Davis, One shields 
            Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 13717)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13717
                     /organism="Cloning vector pPIPRA589"
                     /mol_type="other DNA"
                     /db_xref="taxon:1167440"
     regulatory      16..419
                     /regulatory_class="promoter"
                     /note="MAS promoter"
     promoter        26..406
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             420..1142
                     /codon_start=1
                     /gene="ipt"
                     /product="isopentenyl phosphotransferase"
                     /label=ipt
                     /note="condensation of AMP and isopentenylpyrophosphate to 
                     form isopentenyl-AMP, a cytokinin"
                     /protein_id="AFI23682.1"
                     /translation="MDLHLIFGPTCTGKTTTAIALAQQTGLPVLSLDRVQCCPQLSTGS
                     GRPTVEELKGTTRLYLDDRPLVEGIIAAKQAHHRLIEEVYNHEANGGLILEGGSTSLLN
                     CMARNSYWSADFRWHIIRHKLPDQETFMKAAKARVKQMLHPAAGHSIIQELVYLWNEPR
                     LRPILKEIDGYRYAMLFASQNQITADMLLQLDANMEGKLINGIAQEYFIHARQQEQKFP
                     QVNAAAFDGFEGHPFGMY"
     gene            420..1142
                     /gene="ipt"
                     /label=ipt
     regulatory      1488..1772
                     /regulatory_class="terminator"
                     /note="Mas term 3 UTR"
     terminator      1505..1757
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    1794..2118
                     /label=T-DNA left Border
                     /note="T-DNA left Border"
     misc_feature    2026..2050
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      2137..3080
                     /regulatory_class="promoter"
                     /note="FMV34S"
     CDS             3093..3890
                     /codon_start=1
                     /gene="NPTII"
                     /product="neomycin phosphotransferase II"
                     /label=NPTII
                     /note="confers kanamycin resistance in plants"
                     /protein_id="AFI23683.1"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3093..3890
                     /gene="NPTII"
                     /label=NPTII
     regulatory      3897..4173
                     /regulatory_class="terminator"
                     /note="Mas term 3 UTR"
     terminator      3908..4160
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      4217..5160
                     /regulatory_class="promoter"
                     /note="FMV34S"
     CDS             5227..6510
                     /codon_start=1
                     /gene="codA"
                     /product="cytosine deaminase"
                     /label=codA
                     /note="negative selection marker"
                     /protein_id="AFI23684.1"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITEN
                     SLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQR
                     AWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSY
                     PNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDE
                     QSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNI
                     HLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQ
                     LMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSV
                     RGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            5227..6510
                     /gene="codA"
                     /label=codA
     regulatory      6523..6826
                     /regulatory_class="terminator"
                     /note="Pea E9 3 UTR"
     misc_feature    6857..7050
                     /label=T-DNA right Border
                     /note="T-DNA right Border"
     misc_feature    6913..6937
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7222..10981
                     /label=Agrobacterium pVS1
                     /note="Agrobacterium pVS1"
     CDS             8401..9030
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9459..10532
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10598..10792
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      11119..12256
                     /label=E. coli ColE1
                     /note="E. coli ColE1"
     misc_feature    11266..11406
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11592..12180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12645..13439)
                     /codon_start=1
                     /gene="aadA"
                     /product="AadA"
                     /label=aadA
                     /note="confers kanamycin resistance in bacteria"
                     /protein_id="AFI23685.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(12645..13439)
                     /gene="aadA"
                     /label=aadA